Evaluation of a new enzyme-linked immunosorbent assay for the diagnosis of tuberculosis in goat milk

Caprine tuberculosis (TB) is a zoonosis with sanitary and economic repercussions. Caprine TB control programs are based on a test and cull strategy using the intradermal tuberculin tests and slaughterhouse surveillance. However, this approach is not always feasible and may have a limited sensitivity...

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Bibliographic Details
Published in:Research in veterinary science 2020-02, Vol.128, p.217-223
Main Authors: Roy, A., Infantes-Lorenzo, J.A., Domínguez, M., Moreno, I., Pérez, M., García, N., García-Seco, T., Álvarez, J., Romero, B., Gortázar, C., de Juan, L., Domínguez, L., Bezos, J.
Format: Article
Language:English
Subjects:
Abattoirs
Animals
Antigens
Blood - immunology
Cattle
Cell culture
Control programs
Diagnosis
Diagnostic systems
Diagnostic tests
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - veterinary
Female
Goat
Goat Diseases - diagnosis
Goat Diseases - epidemiology
Goat's milk
Goats
Immunoglobulins
Infections
Milk
Milk - immunology
P22
p22 Protein
Pasteurization
Qualitative analysis
Sensitivity
Sensitivity and Specificity
Serologic Tests - veterinary
Serology
Tuberculin
Tuberculin Test - veterinary
Tuberculosis
Tuberculosis - diagnosis
Tuberculosis - epidemiology
Tuberculosis - veterinary
Vaccines
Veterinary medicine
Zoonoses
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Summary:Caprine tuberculosis (TB) is a zoonosis with sanitary and economic repercussions. Caprine TB control programs are based on a test and cull strategy using the intradermal tuberculin tests and slaughterhouse surveillance. However, this approach is not always feasible and may have a limited sensitivity under specific circumstances. In this study, performance of a new experimental test based on the P22 protein complex (P22 ELISA) was evaluated in two TB-infected herds using milk and serum samples and compared with cell-based diagnostic tests. Samples from a low (n = 62, herd 1) and a high (n = 52, herd 2) TB prevalence herd were selected. Moreover, bulk tank milk samples from both herds were analysed using the P22 ELISA. At the end of the study, a group of animals (n = 21) was euthanized and subjected to post-mortem analysis and bacteriological culture. Significant differences (p 
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2019.12.009