Cryopreservation induces higher oxidative stress levels in Bos indicus embryos compared with Bos taurus

Freezing and thawing of Bos indicus embryos affect their quality for embryo transfer. The objective of this study was to compare the levels of reactive oxygen species between Bos indicus and Bos taurus embryos produced in vivo, before and after conventional freezing, as well as to analyze damage cau...

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Published in:Theriogenology 2020-02, Vol.143, p.74-81
Main Authors: López-Damián, Eva Patricia, Jiménez-Medina, José Alfredo, Alarcón, Marco Antonio, Lammoglia, Miguel Angel, Hernández, Adalinda, Galina, Carlos S., Fiordelisio, Tatiana
Format: Article
Language:English
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Bos indicus
Bos taurus
Embryo preservation
Embryo transfer
Freezing
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cited_by cdi_FETCH-LOGICAL-c386t-bcc17027493f2132fd1395f0835cbe5b3e253404f9b3b7aeed7a20288c8160d23
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container_title Theriogenology
container_volume 143
creator López-Damián, Eva Patricia
Jiménez-Medina, José Alfredo
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Galina, Carlos S.
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description Freezing and thawing of Bos indicus embryos affect their quality for embryo transfer. The objective of this study was to compare the levels of reactive oxygen species between Bos indicus and Bos taurus embryos produced in vivo, before and after conventional freezing, as well as to analyze damage caused by apoptosis and lipid peroxidation. Bos indicus has higher levels of reactive oxygen species than Bos taurus embryos, both fresh (14.32 ± 1.41 auf vs 8.07 ± 1.15 auf (arbitrary units of fluorescence), P 
doi_str_mv 10.1016/j.theriogenology.2019.12.001
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The objective of this study was to compare the levels of reactive oxygen species between Bos indicus and Bos taurus embryos produced in vivo, before and after conventional freezing, as well as to analyze damage caused by apoptosis and lipid peroxidation. Bos indicus has higher levels of reactive oxygen species than Bos taurus embryos, both fresh (14.32 ± 1.41 auf vs 8.07 ± 1.15 auf (arbitrary units of fluorescence), P < 0.05) and after freezing (20.91 ± 1.21 auf vs 14.39 ± 0.58 auf, P < 0.05). The number of apoptotic nuclei is also significantly higher in Bos indicus embryos than Bos taurus (8.28 ± 0.80 vs 1 ± 0.57, P < 0.05) but highlighting a notable increase after the freeze-thaw process in both subspecies (Bos indicus from 8.28 ± 0.80 to 10.71 ± 0.42, P < 0.05; Bos taurus from 1 ± 0.57 to 5.5 ± 1.15, P < 0.05). Finally, although lipid peroxidation is lower in Bos indicus embryos before freezing in comparison with Bos taurus (2.46 ± 0.14 vs 4.20 ± 0.51), the effect after the freeze-thaw process showed an increase of 4.34 in Bos indicus than Bos taurus embryos (51.45 ± 5.52 auf vs 11.85 ± 2.88 auf, P < 0.05). In conclusion, in comparison with Bos taurus, Bos indicus embryos undergo greater oxidative stress causing increases in the cryopreservation process, promoting major cell damage and lowering embryonic viability. •B. indicus embryos have more ROS than B. taurus before and after freezing.•B. indicus embryos have more apoptotic nuclei than B. taurus before and after freezing.•Cryopreservation dramatically increased lipid peroxidation in B. indicus embryos.]]></description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2019.12.001</identifier><identifier>PMID: 31838342</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Bos indicus ; Bos taurus ; Embryo preservation ; Embryo transfer ; Freezing</subject><ispartof>Theriogenology, 2020-02, Vol.143, p.74-81</ispartof><rights>2019 Elsevier Inc.</rights><rights>Copyright © 2019 Elsevier Inc. 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The objective of this study was to compare the levels of reactive oxygen species between Bos indicus and Bos taurus embryos produced in vivo, before and after conventional freezing, as well as to analyze damage caused by apoptosis and lipid peroxidation. Bos indicus has higher levels of reactive oxygen species than Bos taurus embryos, both fresh (14.32 ± 1.41 auf vs 8.07 ± 1.15 auf (arbitrary units of fluorescence), P < 0.05) and after freezing (20.91 ± 1.21 auf vs 14.39 ± 0.58 auf, P < 0.05). The number of apoptotic nuclei is also significantly higher in Bos indicus embryos than Bos taurus (8.28 ± 0.80 vs 1 ± 0.57, P < 0.05) but highlighting a notable increase after the freeze-thaw process in both subspecies (Bos indicus from 8.28 ± 0.80 to 10.71 ± 0.42, P < 0.05; Bos taurus from 1 ± 0.57 to 5.5 ± 1.15, P < 0.05). Finally, although lipid peroxidation is lower in Bos indicus embryos before freezing in comparison with Bos taurus (2.46 ± 0.14 vs 4.20 ± 0.51), the effect after the freeze-thaw process showed an increase of 4.34 in Bos indicus than Bos taurus embryos (51.45 ± 5.52 auf vs 11.85 ± 2.88 auf, P < 0.05). In conclusion, in comparison with Bos taurus, Bos indicus embryos undergo greater oxidative stress causing increases in the cryopreservation process, promoting major cell damage and lowering embryonic viability. •B. indicus embryos have more ROS than B. taurus before and after freezing.•B. indicus embryos have more apoptotic nuclei than B. taurus before and after freezing.•Cryopreservation dramatically increased lipid peroxidation in B. indicus embryos.]]></description><subject>Bos indicus</subject><subject>Bos taurus</subject><subject>Embryo preservation</subject><subject>Embryo transfer</subject><subject>Freezing</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqNkD1v2zAQhokiReOk_QsFhw5ZpPBIS6KALK3RfAABurRAN0IiTzYNSXR5klP_-9J1UiBbpxvuee_FPYx9ApGDgPJ6m08bjD6scQx9WB9yKaDOQeZCwBu2AF3VmZIKzthCiFplZQ0_z9kF0VYIocoS3rFzBVpptZQLtl7FQ9hFJIz7ZvJh5H50s0XiG79OPTz89i4t9shpShjxHvfYU8L4l3AcztuZOA5tOkTchmHXRHT8yU-bv8TUzHGm9-xt1_SEH57nJftx-_X76j57_Hb3sPr8mFmlyylrrYVKyGpZq06Ckp0DVRed0KqwLRatQlmopVh2davaqkF0VSOF1NpqKIWT6pJdne7uYvg1I01m8GSx75sRw0xGKlml1wutE3pzQm0MRBE7s4t-aOLBgDBH1WZrXqs2R9UGpEmqU_zjc9PcDuj-hV_cJuD2BCRduPcYDVmPo0XnI9rJuOD_r-kPoXaa3w</recordid><startdate>202002</startdate><enddate>202002</enddate><creator>López-Damián, Eva Patricia</creator><creator>Jiménez-Medina, José Alfredo</creator><creator>Alarcón, Marco Antonio</creator><creator>Lammoglia, Miguel Angel</creator><creator>Hernández, Adalinda</creator><creator>Galina, Carlos S.</creator><creator>Fiordelisio, Tatiana</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202002</creationdate><title>Cryopreservation induces higher oxidative stress levels in Bos indicus embryos compared with Bos taurus</title><author>López-Damián, Eva Patricia ; Jiménez-Medina, José Alfredo ; Alarcón, Marco Antonio ; Lammoglia, Miguel Angel ; Hernández, Adalinda ; Galina, Carlos S. ; Fiordelisio, Tatiana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-bcc17027493f2132fd1395f0835cbe5b3e253404f9b3b7aeed7a20288c8160d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Bos indicus</topic><topic>Bos taurus</topic><topic>Embryo preservation</topic><topic>Embryo transfer</topic><topic>Freezing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>López-Damián, Eva Patricia</creatorcontrib><creatorcontrib>Jiménez-Medina, José Alfredo</creatorcontrib><creatorcontrib>Alarcón, Marco Antonio</creatorcontrib><creatorcontrib>Lammoglia, Miguel Angel</creatorcontrib><creatorcontrib>Hernández, Adalinda</creatorcontrib><creatorcontrib>Galina, Carlos S.</creatorcontrib><creatorcontrib>Fiordelisio, Tatiana</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>López-Damián, Eva Patricia</au><au>Jiménez-Medina, José Alfredo</au><au>Alarcón, Marco Antonio</au><au>Lammoglia, Miguel Angel</au><au>Hernández, Adalinda</au><au>Galina, Carlos S.</au><au>Fiordelisio, Tatiana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryopreservation induces higher oxidative stress levels in Bos indicus embryos compared with Bos taurus</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2020-02</date><risdate>2020</risdate><volume>143</volume><spage>74</spage><epage>81</epage><pages>74-81</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract><![CDATA[Freezing and thawing of Bos indicus embryos affect their quality for embryo transfer. The objective of this study was to compare the levels of reactive oxygen species between Bos indicus and Bos taurus embryos produced in vivo, before and after conventional freezing, as well as to analyze damage caused by apoptosis and lipid peroxidation. Bos indicus has higher levels of reactive oxygen species than Bos taurus embryos, both fresh (14.32 ± 1.41 auf vs 8.07 ± 1.15 auf (arbitrary units of fluorescence), P < 0.05) and after freezing (20.91 ± 1.21 auf vs 14.39 ± 0.58 auf, P < 0.05). The number of apoptotic nuclei is also significantly higher in Bos indicus embryos than Bos taurus (8.28 ± 0.80 vs 1 ± 0.57, P < 0.05) but highlighting a notable increase after the freeze-thaw process in both subspecies (Bos indicus from 8.28 ± 0.80 to 10.71 ± 0.42, P < 0.05; Bos taurus from 1 ± 0.57 to 5.5 ± 1.15, P < 0.05). Finally, although lipid peroxidation is lower in Bos indicus embryos before freezing in comparison with Bos taurus (2.46 ± 0.14 vs 4.20 ± 0.51), the effect after the freeze-thaw process showed an increase of 4.34 in Bos indicus than Bos taurus embryos (51.45 ± 5.52 auf vs 11.85 ± 2.88 auf, P < 0.05). In conclusion, in comparison with Bos taurus, Bos indicus embryos undergo greater oxidative stress causing increases in the cryopreservation process, promoting major cell damage and lowering embryonic viability. •B. indicus embryos have more ROS than B. taurus before and after freezing.•B. indicus embryos have more apoptotic nuclei than B. taurus before and after freezing.•Cryopreservation dramatically increased lipid peroxidation in B. indicus embryos.]]></abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>31838342</pmid><doi>10.1016/j.theriogenology.2019.12.001</doi><tpages>8</tpages></addata></record>
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subjects Bos indicus
Bos taurus
Embryo preservation
Embryo transfer
Freezing
title Cryopreservation induces higher oxidative stress levels in Bos indicus embryos compared with Bos taurus
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