Integrated Microstructured Photonic Fiber as a Bifunctional Robust Frit and Efficient Electrospray Emitter of a Packed Column for Capillary Liquid Chromatography–Tandem Mass Spectrometry Analysis of Complex Biological Samples

Although capillary liquid chromatography married with tandem mass spectrometry (cLC–MS/MS) has become a powerful technique for proteomics and metabolomics research, it is still a great challenge to fabricate durable capillary-based analytical columns coupling continuous nanoflow (100), which always...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2020-01, Vol.92 (2), p.2274-2282
Main Authors: Ma, Shujuan, Wang, Yan, Zhang, Na, Lyu, Jiawen, Ma, Chen, Xu, Junwen, Li, Xiaowei, Ou, Junjie, Ye, Mingliang
Format: Article
Language:English
Subjects:
Alkylbenzenes
Biological properties
Biological samples
Boring
Capillaries
Chemistry
Chromatography
Chromatography, Liquid
Column chromatography
Coupling (molecular)
Electrospraying
Emitters
Fibers
Frit
HeLa Cells
Humans
Integrals
Ionization
Ions
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metabolomics
Microchannels
Optical Fibers
Packed columns
Particle Size
Peptides
Peptides - analysis
Photonics
Photons
Polymers
Proteins - analysis
Proteomics
Scientific imaging
Silica
Silicon dioxide
Spectrometry, Mass, Electrospray Ionization
Spectroscopy
Surface Properties
Tandem Mass Spectrometry
Tumor Cells, Cultured
Ultraviolet Rays
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Summary:Although capillary liquid chromatography married with tandem mass spectrometry (cLC–MS/MS) has become a powerful technique for proteomics and metabolomics research, it is still a great challenge to fabricate durable capillary-based analytical columns coupling continuous nanoflow (100), which always encumbers both independent and integral tapered tip emitters for cLC–MS.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b04997