A metal organic framework-functionalized monolithic column for enantioseparation of six basic chiral drugs by capillary electrochromatography

• Published in: Mikrochimica acta (1966) 2020-01, Vol.187 (1), p.51-51, Article 51
• Main Authors: Ding, Wen, Yu, Tao, Du, Yingxiang, Sun, Xiaodong, Feng, Zijie, Zhao, Shiyuan, Ma, Xiaofei, Ma, Mingxuan, Chen, Cheng
• Format: Article
• Language: English
• Subjects: Analysis; Analytical Chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatography; Diffraction; Ethylene; Infrared spectroscopy; Microengineering; Nanochemistry; Nanotechnology; Original Paper; X-rays
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-019-3998-0

Poly(glycidyl methacrylate)-co-(ethylene dimethacrylate) [poly(GMA-co-EDMA)] monoliths were used as a support to grow a zeolitic imidazolate framework-8 (ZIF-8) via layer-by-layer self-assembly. Pepsin, acting as as chiral selector, was covalently linked to the surface of the amino-modified ZIF-8 through the Schiff base method. The material was characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Fourier transform infrared spectroscopy and elemental analysis. The pepsin-ZIF-8-poly(GMA-co-EDMA) column was utilized to the enantioseparation of the racemic forms of hydroxychloroquine (HCQ), chloroquine (CHQ), hydroxyzine (HXY), nefopam (NEF), clenbuterol (CLE) and amlodipine (AML). In comparison with a pepsin-poly(GMA-co-EDMA) monolithic column (without self-assembled ZIF-8 nanoparticles), the resolution is strongly enhanced (HCQ: 0.34 → 2.50; CHQ: 0.45 → 1.97; HXY: 0.39 → 1.43; NEF: 0.27 → 0.81; CLE: 0 → 0.81; AML: 0.16 → 0.72). Effects of self-assembly layers of ZIF-8, pepsin concentration, buffer pH values and applied voltage were investigated with hydroxychloroquine as the model analyte. The reproducibility of run-to-run, day-to-day and column-to-column were explored, and found to be satisfactory. Graphical abstract Schematic representation of capillary electrochromatography (CEC) systems with a pepsin-zeolitic imidazolate framework-8 (ZIF-8) modified poly(glycidyl methacrylate)-co-(ethylene dimethacrylate) [poly(GMA-co-EDMA)] monolithic column as stationary phases for separation of basic racemic drugs. ZIF-8 modified column was prepared via layer-by-layer self-assembly.