Interleukin-18 and its gene single nucleotide polymorphisms (SNPs) influence chronic hepatitis C progression

• Published in: Journal of infection in developing countries 2018-04, Vol.12 (4), p.257-264
• Main Authors: Said, Ebada M, Soliman, Mohamed S, Shousha, Hend Ibrahim, Rashed, Mohamed Sanad, Elazm, Abeer Abo, Aamer, Rania Zakaria, Kamel, Mai Hamed, Abdelsalam, Fatma Mohamed
• Format: Article
• Language: English
• Subjects: Cytokines; Gene expression; Hepatitis C; Liver diseases; Plasma
• ISSN: 1972-2680; 2036-6590; 1972-2680
• DOI: 10.3855/jidc.9813

Interleukin-18 (IL-18) is a pro-inflammatory cytokine that is induced by hepatitis C virus (HCV) infection. Inter-individual variations of IL-18 gene expression may alter HCV-associated liver injury. Variable single nucleotide polymorphisms (SNPs) have been detected within IL-18 gene sequence. Quantitative assessment of IL-18 plasma level and detection of genotype frequencies of 2 functional polymorphisms of its gene (-607 C/A and -137 G/C) were done to assess their impact on the severity of chronic hepatitis C (CHC). Cases group (I) comprised 110 treatment naïve CHC Egyptian patients (78 Males and 32 Females, mean age = 40.7 ± 11.8 years) who underwent routine laboratory investigations. Assessment of plasma level of IL-18 was done by enzyme-linked immunosorbent assay (ELISA), detection of IL-18 gene polymorphisms at positions -607 C/A and -137 G/C by polymerase chain reaction sequence specific polymorphism (PCR-SSP) analysis and Liver biopsy with METAVIR scoring were done. The control group (II) comprised 90 healthy participants. Plasma levels of IL-18 were significantly higher in cases than the control group. We found a statistically highly significant (p < 0.001) positive correlation between IL-18 plasma level and both METAVIR necro-inflammatory grade and fibrosis stage. The A/A allele at -607 position was significantly more frequent (p < 0.05) in patients with F ≤ 1. Higher IL-18 plasma levels are found in CHC patients and positively correlate with the severity of liver disease. The presence of A/A allele at -607 position of IL-18 gene promoter is associated with milder liver disease.