Resveratrol Downregulates STAT3 Expression and Astrocyte Activation in Primary Astrocyte Cultures of Rat

Astrocytes respond to all forms of central nervous system (CNS) insults by a process referred to as reactive astrogliosis. Inhibition of astrocyte growth and activation is an important strategy for promoting injured CNS repair. STAT3 (signal transducer and activator of transcription 3) is reported t...

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Bibliographic Details
Published in:Neurochemical research 2020-02, Vol.45 (2), p.455-464
Main Authors: Wu, Moli, Wang, Lihong, Li, Fengzhi, Hu, Ruina, Ma, Jingxin, Zhang, Kaili, Cheng, Xiaoxin
Format: Article
Language:English
Subjects:
Activation
Astrocytes
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell proliferation
Central nervous system
Deactivation
Diet
Glial fibrillary acidic protein
Gliosis
Inhibitors
Injuries
Kinases
Neurochemistry
Neurology
Neurosciences
Original Paper
Phosphorylation
Resveratrol
Scratching
Stat3 protein
Transcription
Wounds
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Summary:Astrocytes respond to all forms of central nervous system (CNS) insults by a process referred to as reactive astrogliosis. Inhibition of astrocyte growth and activation is an important strategy for promoting injured CNS repair. STAT3 (signal transducer and activator of transcription 3) is reported to be a critical regulator of astrogliosis, and resveratrol (RES, a dietary polyphenol) is considered to be a natural inhibitor of STAT3 expression and phosphorylation. In this study, we investigated the effects of RES on STAT3 expression and phosphorylation, and then on the proliferation and activation of astrocytes, a critical process in reactive astrogliosis, in rat primary cultured astrocytes and an in vitro scratch-wound model. RES downregulated the expression levels of STAT3, P-STAT3 and GFAP (glial fibrillary acidic protein) in cultured astrocytes. The positive index of Ki67 was apparently reduced in cultured astrocytes after RES treatment. Meanwhile, cultured astrocyte proliferation and activation were attenuated by RES. Moreover, in the established in vitro scratch-wound model the increased expression levels of STAT3, P-STAT3 and GFAP induced by scratching injury were also clearly inhibited by RES. In addition, the inhibitory effect of RES on cell proliferation was similar to that of AG490 (a selective inhibitor of STAT3 phosphorylation) and abrogated by Colivelin (a STAT3 activator) stimuli. Taken together, our data suggest that RES is able to inhibit reactive astrocyte proliferation and activation mainly via deactivating STAT3 pathway. So RES may have a therapeutic benefit for the treatment of the injured CNS.
ISSN:0364-3190
1573-6903
DOI:10.1007/s11064-019-02936-9