Transposing Lateral Flow Immunoassays to Capillary-Driven Microfluidics Using Self-Coalescence Modules and Capillary-Assembled Receptor Carriers

Point-of-care (POC) immunodiagnostic tests play a crucial role in enabling rapid and correct diagnosis of diseases in prehospital care, emergency, and remote settings. In this work, we present a silicon-based, capillary-driven microfluidic chip integrating two microfluidic modules for the implementa...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2020-01, Vol.92 (1), p.940-946
Main Authors: Hemmig, Elisa, Temiz, Yuksel, Gökçe, Onur, Lovchik, Robert D, Delamarche, Emmanuel
Format: Article
Language:English
Subjects:
Antibodies
Antibodies, Immobilized - immunology
Antibodies, Monoclonal - immunology
Cabs
Calcium-binding protein
Capillary flow
Carbocyanines - chemistry
Chemistry
Coalescence
Coalescing
Fluorescent Dyes - chemistry
Humans
Immunoassay
Immunoassay - instrumentation
Immunoassay - methods
Immunoassays
Lab-On-A-Chip Devices
Limit of Detection
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microfluidics
Microspheres
Modules
Nanoparticles
Point-of-Care Testing
Reagents
Receptors
Self-assembly
Troponin
Troponin I
Troponin I - blood
Troponin I - immunology
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Summary:Point-of-care (POC) immunodiagnostic tests play a crucial role in enabling rapid and correct diagnosis of diseases in prehospital care, emergency, and remote settings. In this work, we present a silicon-based, capillary-driven microfluidic chip integrating two microfluidic modules for the implementation of highly miniaturized immunoassays. Specifically, we apply state-of-the-art microfluidic technology to demonstrate a one-step immunoassay for the detection of the cardiac marker troponin I in human serum using sample volumes of ∼1 μL and with a limit of detection (LOD) of ∼4 ng mL–1 within 25 min. The microfluidic modules discussed here broadly map functionalities found in standard lateral flow assays. We implement a self-coalescence module (SCM) for the controlled reconstitution and delivery of inkjet-spotted and dried detection antibodies (dAbs). This allows for homogeneous dissolution of 1.3 ng of fluorescently labeled dAbs in 416 nL of the sample used for the assay. We also show how to immobilize receptors inside closed microfluidic devices in
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b03792