Integrated Analysis of Comparative Lipidomics and Proteomics Reveals the Dynamic Changes of Lipid Molecular Species in High-Oleic Acid Peanut Seed

• Published in: Journal of agricultural and food chemistry 2020-01, Vol.68 (1), p.426-438
• Main Authors: Liu, Hao, Hong, Yanbin, Lu, Qing, Li, Haifen, Gu, Jianzhong, Ren, Li, Deng, Li, Zhou, Baojin, Chen, Xiaoping, Liang, Xuanqiang
• Format: Article
• Language: English
• Subjects: Arachis - chemistry; Arachis - genetics; Arachis - growth & development; Arachis - metabolism; Fatty Acid Desaturases - genetics; Fatty Acid Desaturases - metabolism; Lipid Metabolism; Lipidomics; Lipids - chemistry; Mutation; Oleic Acid - chemistry; Oleic Acid - metabolism; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Proteomics; Seeds - chemistry; Seeds - genetics; Seeds - growth & development; Seeds - metabolism
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.9b04179

Modern peanut contains fatty acid desaturase 2 (FAD2) mutation, which is capable of producing high oleic acid for human health. However, the dynamic changes of the lipidome regarding fad2 remain elusive in peanut seed. In the present study, 547 lipid features were identified in high- and normal-oleic peanut seeds by utilizing the mass spectrometric approach. The fad2-induced differently expressed lipids (DELs) were polarly distributed at early and maturation stages during high-oleic acid (OA) seed development. Subsequently, integration of previously published proteomic data and lipidomic data revealed that 21 proteins and 149 DELs were annotated into the triacylglycerol assembly map, of which nine enzymes and 31 lipid species shared similar variation tendencies. Additionally, the variation tendencies of 17 acyl fatty acids were described in a hypothetical biosynthetic pathway. Collectively, the understanding of the lipid composition correlated with fad2 established a foundation for future high-OA peanut breeding based on lipidomic data.