Comparative mitochondrial genomic analysis of Macrophthalmus pacificus and insights into the phylogeny of the Ocypodoidea & Grapsoidea

• Published in: Genomics (San Diego, Calif.) Calif.), 2020-01, Vol.112 (1), p.82-91
• Main Authors: Wang, Qi, Tang, Dan, Guo, Huayun, Wang, Jie, Xu, Xinyi, Wang, Zhengfei
• Format: Article
• Language: English
• Subjects: Grapsoidea; Macrophthalmus pacificus; Mitogenome; Ocypodoidea; Phylogenetics
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2019.12.012

Grapsoidea and Ocypodoidea, two of the most abundant and economically important groups in Brachyura, are of great commercial value to fisheries and aquaculture. However, the taxonomy of Ocypodoidea and Grapsoidea has long been highly disputed. Previous studies have investigated this problem through phylogenetic analysis based on limited taxonomic sampling, with different reports proposing either monophyly or paraphyly, but no definitive conclusion has been reached. In this study, the complete mitogenome of Macrophthalmus pacificus (Ocypodoidea, Macrophthalmidae) is reported on and the relationship between Ocypodoidea and Grapsoidea is further investigated. Sequencing the M. pacificus mitogenome, which is a closed circular molecule containing a typical 37 genes, preliminarily determined the ancestral gene order of Macrophthalmidae, which is consistent with previous studies. Comparative analyses of gene order among Ocypodoidea and Grapsoidea revealed that Varunidae (Grapsoidea) and Macrophthalmidae (Ocypodoidea) have the same rearrangement, which confirms previous research. Larger data analysis revealed that these two families (Varunidae and Macrophthalmidae) cluster into a monophyletic clade as sister groups. Rearrangement and phylogeny lines of evidence is concluded that Varunidae and Macrophthalmidae may be of common origin. Furthermore, the remaining Ocypodoidea and Grapsoidea families mix paraphyletically in the phylogenetic tree. Therefore, both gene rearrangement and phylogenetic analysis support the paraphyly of Ocypodoidea and Grapsoidea, which reinforces this view. These findings provide important information regarding Brachyura's phylogenetic relationships, which demonstrates the advantage of mitogenome sequence data in phylogenetic studies. •The complete mitogenome of Macrophthalmus pacificus was firstly determined and analyzed.•Phylogenetic analysis supports the placement of Macrophthalmus pacificus in the Macrophthalmidae.•Varunidae and Macrophthalmidae, which are from different superfamilies, may be of common origin.•Both gene rearrangement and phylogenetic analysis support the paraphyly of Ocypodoidea and Grapsoidea.