Production of generic monoclonal antibody and development of chemiluminescence immunoassay for determination of 32 sulfonamides in chicken muscle

•A monoclonal antibody capable of recognizing 32 sulfonamides was first produced.•Recognition differences for the 32 drugs were studied by computation simulation.•A heterologous CL-ELISA was developed for detection of the 32 drugs in chicken. A hapten of sulfabenzamide was first synthesized to gener...

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Bibliographic Details
Published in:Food chemistry 2020-05, Vol.311, p.125966-125966, Article 125966
Main Authors: Li, Zhao Bin, Cui, Peng Lei, Liu, Jing, Liu, Ju Xiang, Wang, Jian Ping
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Chemiluminescence
Chickens - metabolism
Computational simulation
Enzyme linked immunosorbent assay
Food Contamination - analysis
Haptens - chemistry
Immunoassay - methods
Luminescent Measurements
Meat
Monoclonal antibody
Muscles - chemistry
Muscles - metabolism
Sulfonamides
Sulfonamides - analysis
Sulfonamides - chemistry
Sulfonamides - immunology
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Summary:•A monoclonal antibody capable of recognizing 32 sulfonamides was first produced.•Recognition differences for the 32 drugs were studied by computation simulation.•A heterologous CL-ELISA was developed for detection of the 32 drugs in chicken. A hapten of sulfabenzamide was first synthesized to generate a monoclonal antibody that simultaneously recognized 32 sulfonamides. The computational simulation showed that the 3D conformation, molecular bend angle, molecular volume, electronic charge of core structure of these drugs all showed influences on the antibody binding. The antibody was combined with a heterologous enzyme-labeled hapten to develop a direct competitive chemiluminescence enzyme linked immunosorbent assay for determination of the 32 sulfonamides in chicken muscle sample. The CRs of the optimized method for these drugs were in the range of 7.3%–1778%, and the IC50 values were in the range of 0.038–11.2 ng/g. The limits of detection for detection of these drugs in chicken were in the range of 0.03–26 ng/g. Their recoveries from the standards fortified blank chicken samples were in the range of 60.8%–97.1%. Therefore, this method could be used as a useful tool for routine screening sulfonamides residues in meat.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2019.125966