AGT rs699 and AGTR1 rs5186 gene variants are associated with cardiovascular-related phenotypes in atherosclerotic peripheral arterial obstructive disease

• Published in: Irish journal of medical science 2020-08, Vol.189 (3), p.885-894
• Main Authors: Junusbekov, Yerik, Bayoglu, Burcu, Cengiz, Mujgan, Dirican, Ahmet, Arslan, Caner
• Format: Article
• Language: English
• Subjects: Family Medicine; General Practice; Internal Medicine; Medicine; Medicine & Public Health; Original Article
• ISSN: 0021-1265; 1863-4362
• DOI: 10.1007/s11845-019-02166-6

Background Peripheral arterial diseases (PAD) refer to the arterial diseases other than coronary arteries and the aorta. Atherosclerosis is the major cause of PAD. Renin angiotensin aldosterone system (RAAS)-related genes were associated with cardiovascular diseases. Angiotensin II is the pro-inflammatory, proliferative and vasoconstrictor effector of RAAS in the vascular system. Aims In this study, we aimed to investigate whether the effects of the angiotensinogen ( AGT ) rs699 (M268T), angiotensin-converting enzyme ( ACE ) I/D (rs1799752), angiotensin II receptor type 1 ( AGTR1 ) (A1166C) rs5186, and angiotensin II receptor type 2 ( AGTR2 ) rs35474657 variants were associated with PAD etiology due to atherosclerotic involvement of aorta-iliac and femoro-popliteal artery occlusions. Methods AGT rs699, AGTR1 rs5186, ACE I/D (rs1799752), AGTR2 rs35474657 gene variants were determined by real-time polymerase chain reaction (RT-PCR) in 63 PAD patients (33 femoro-popliteal, 30 aorta-iliac) and 70 healthy controls. Results Although there was no significant relationship in the genotype frequencies of AGT rs699, AGTR1 rs5186, ACE I/D (rs1799752), and AGTR2 rs35474657 variants between PAD and control groups ( p  > 0.05), AGT rs699 TT genotype was significantly associated with fasting glucose ( p  = 0.023) in PAD patients. Besides, CC genotype of rs699 was significantly related with HDL-cholesterol levels ( p  = 0.020) in PAD group. Furthermore, AGTR1 rs5186 CC genotype carriers demonstrated significantly higher LDL-cholesterol ( p  = 0.034) and triglycerides levels ( p  = 0.007). Conclusions This report is the first to show an association between RAAS-related gene variants and their relation with the biochemical characteristics of PAD and suggests that RAAS-associated gene variants may have significant roles in cardiovascular related phenotypes of PAD patients.