Highly uniform in-situ cell electrotransfection of adherent cultures using grouped interdigitated electrodes

•Uniform electrotransfection obtained by sequential energizing of two electrode pairs.•Cells exposed to an overall equal intensity of electric field.•Seven different cell types successfully transfected.•Higher transfection efficiency and cell viability achieved. Cell electrotransfection is an effect...

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Bibliographic Details
Published in:Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2020-04, Vol.132, p.107435-107435, Article 107435
Main Authors: Zhou, Yicen, Lu, Ying, Cheng, Jing, Xu, Youchun
Format: Article
Language:English
Subjects:
Activation
Adherent cells
Cell Adhesion
Cell lines
Cell Survival
Cell viability
Efficiency
Electric contacts
Electric fields
Electrodes
Electroporation - methods
Electrotransfection
HeLa Cells
Humans
In-situ electrotransfection
Transfection
Transfection - methods
Transfection efficiency
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Summary:•Uniform electrotransfection obtained by sequential energizing of two electrode pairs.•Cells exposed to an overall equal intensity of electric field.•Seven different cell types successfully transfected.•Higher transfection efficiency and cell viability achieved. Cell electrotransfection is an effective approach for transferring exogenous molecules into living cells by electric stimulation. The existing in-situ electrotransfection micro-devices for adherent cells exhibit the drawbacks of low transfection efficiency and low cell viability. An important reason for these drawbacks is the unequal exposure of cells to the electric field. It was found that cells growing directly below the energized electrodes experience a much lower electric field intensity when compared to the cells growing below the spacing area of the electrodes, resulting in low transfection with a strip-like pattern. Therefore, a new strategy for the in-situ electrotransfection of adherent cells growing in a standard 12-well plate is proposed in this study. By sequentially energizing electrodes arranged in a nested and non-contact manner, the cells were exposed to an overall equal intensity of the electric field, and thus a higher efficiency of transfection was achieved. The seven cell lines transfected using this method exhibited high transfection efficiency and high cell viability, demonstrating the potential for studying gene function.
ISSN:1567-5394
1878-562X
DOI:10.1016/j.bioelechem.2019.107435