Enhanced β-glucan and pullulan production by Aureobasidium pullulans with zinc sulfate supplementation

The effects of mineral salts on the production of exopolysaccharides, including β -glucan and pullulan, by Aureobasidium pullulans CCTCC M 2012259 were investigated. Zinc sulfate at certain concentrations decreased dry biomass but favored to the biosynthesis of both exopolysaccharides. When 100 mg/L...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2020-02, Vol.104 (4), p.1751-1760
Main Authors: Wang, D., Zhu, C., Zhang, G., Wang, C., Wei, G.
Format: Article
Language:English
Subjects:
Applied Microbial and Cell Physiology
Aureobasidium pullulans
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Energy metabolism
Enzymes
Exopolysaccharides
Fermentation
Fungi
Gene expression
Glucan
Intracellular
Life Sciences
Microbial Genetics and Genomics
Microbiology
Physiology
Pullulan
Salts
Sulfates
Supplements
Transcription
Uridine
Zinc
Zinc sulfate
β-Glucan
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Summary:The effects of mineral salts on the production of exopolysaccharides, including β -glucan and pullulan, by Aureobasidium pullulans CCTCC M 2012259 were investigated. Zinc sulfate at certain concentrations decreased dry biomass but favored to the biosynthesis of both exopolysaccharides. When 100 mg/L zinc sulfate was added to the fermentation medium, production of β -glucan and pullulan increased by 141.7 and 10.2%, respectively, when compared with that noted in the control without zinc sulfate addition. To reveal the physiological mechanism underlying improved β -glucan and pullulan production, key enzymes activities, energy metabolism substances, intracellular uridine diphosphate glucose (UDPG) levels, and gene expression were determined. The results indicated that zinc sulfate up-regulated the transcriptional levels of pgm1 , ugp , fks , and kre6 genes, increased activities of key enzymes involved in the biosynthesis of UDPG, β -glucan and pullulan, enhanced intracellular UDPG content, and improved energy supply, all of which contributed to the increment in β -glucan and pullulan production. The present study not only provides a feasible approach to improve the production of exopolysaccharides but also contributes to better understanding of the physiological characteristics of A. pullulans .
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-019-10326-y