Genotypic and symbiotic diversity of native rhizobia nodulating red pea (Lathyrus cicera L.) in Tunisia

Nodulation and genetic diversity of native rhizobia nodulating Lathyrus cicera plants grown in 24 cultivated and marginal soils collected from northern and central Tunisia were studied. L. cicera plants were nodulated and showed the presence of native rhizobia in 21 soils. A total of 196 bacterial s...

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Bibliographic Details
Published in:Systematic and applied microbiology 2020-01, Vol.43 (1), p.126049-126049, Article 126049
Main Authors: Gritli, Takwa, Ellouze, Walid, Chihaoui, Saif-Allah, Barhoumi, Fathi, Mhamdi, Ridha, Mnasri, Bacem
Format: Article
Language:English
Subjects:
Biodiversity
Biomass
DNA, Bacterial - genetics
Ensifer meliloti
Genes, Bacterial - genetics
Genes, Essential - genetics
Genetic Variation
Genotype
Lathyrus - growth & development
Lathyrus - microbiology
Lathyrus cicero
Molecular diversity
Phylogeny
Plant Root Nodulation - genetics
Plant Root Nodulation - physiology
Rhizobium - classification
Rhizobium - genetics
Rhizobium - isolation & purification
Rhizobium laguerreae
Rhizobium leguminosarum
Root Nodules, Plant - microbiology
Sequence Analysis, DNA
Soil Microbiology
Symbiosis - genetics
Symbiotic diversity
Tunisia
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Summary:Nodulation and genetic diversity of native rhizobia nodulating Lathyrus cicera plants grown in 24 cultivated and marginal soils collected from northern and central Tunisia were studied. L. cicera plants were nodulated and showed the presence of native rhizobia in 21 soils. A total of 196 bacterial strains were selected and three different ribotypes were revealed after PCR-RFLP analysis. The sequence analysis of the rrs and two housekeeping genes (recA and thrC) from 36 representative isolates identified Rhizobium laguerreae as the dominant (53%) rhizobia nodulating L. cicera. To the best of our knowledge, this is the first time that this species has been reported among wild populations of the rhizobia-nodulating Lathyrus genus. Twenty-five percent of the isolates were identified as R. leguminosarum and isolates LS11.5, LS11.7 and LS8.8 clustered with Ensifer meliloti. Interestingly, five isolates (LS20.3, LS18.3, LS19.10, LS1.2 and LS21.20) were segregated from R. laguerreae and clustered as a separate clade. These isolates possibly belong to new species. According to nodC and nodA phylogeny, strains of R. laguerreae and R. leguminosarum harbored the symbiotic genes of symbiovar viciae and clustered in three different clades showing heterogeneity within the symbiovar. Strains of E. meliloti harbored symbiotic genes of Clade V and induced inefficient nodules.
ISSN:0723-2020
1618-0984
DOI:10.1016/j.syapm.2019.126049