Highly sensitive and selective fluorescent monomer/polymer probes for Hg2+ and Ag+ recognition and imaging of Hg2+ in living cells

The detection of heavy metals such as Hg 2+ and Ag + is important and urgent. In this work, – NO 2 /– NH 2 /C=S boron dipyrromethene small molecular derivatives were synthesized at first. Then they were incorporated into polymer chains. The macromolecular fluorescent probes were obtained via Sonogas...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2020-02, Vol.412 (4), p.881-894
Main Authors: Xiao, Li, Sun, Qi, Zhao, Qiang, Cheng, Xinjian
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biochemistry
Boron
Cell membranes
Characterization and Evaluation of Materials
Chemical synthesis
Chemistry
Chemistry and Materials Science
Cytotoxicity
Detection
Electron transfer
Fluorescent indicators
Food Science
Heavy metals
Laboratory Medicine
Macromolecules
Medical imaging
Membrane permeability
Mercury (metal)
Metal ions
Monitoring/Environmental Analysis
Nitrogen dioxide
Polymers
Probes
Recognition
Research Paper
Silver
Toxicity
Zebrafish
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Summary:The detection of heavy metals such as Hg 2+ and Ag + is important and urgent. In this work, – NO 2 /– NH 2 /C=S boron dipyrromethene small molecular derivatives were synthesized at first. Then they were incorporated into polymer chains. The macromolecular fluorescent probes were obtained via Sonogashira reaction using the small molecular probes as building blocks. The as-prepared small-molecule fluorescent probe BO3 exhibits high sensing performance for Hg 2+ . By introducing it into macromolecules, the sensing ability still remains, and even more, the recognition performance is improved. The macromolecular fluorescent probes P1 , P2 , and P3 also have high recognition ability for Ag + with a binding ratio of 2:1 (metal ion to probe ratio). Through the study of the sensing mechanism and the recycling experiments, it is found that the probes responded by the photo-induced electron transfer mechanism and can be recycled and reused. At the same time, BO3 , P2 , and P3 show excellent recognition performance for Hg 2+ in living cells and zebrafish. Living cell imaging experiments indicated that these fluorescent probes had good cell membrane permeability and low cytotoxicity, and could realize bioimaging of Hg 2+ . Therefore, the application value of these fluorescent probes could be enlarged. Graphical abstract
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-019-02297-w