IL-10 delays the degeneration of intervertebral discs by suppressing the p38 MAPK signaling pathway

• Published in: Free radical biology & medicine 2020-02, Vol.147, p.262-270
• Main Authors: Ge, Jun, Yan, Qi, Wang, Yingjie, Cheng, Xiaoqiang, Song, Dawei, Wu, Cenhao, Yu, Hao, Yang, Huilin, Zou, Jun
• Format: Article
• Language: English
• Subjects: Animals; Cells, Cultured; Degeneration; IL-10; Interleukin-10 - genetics; Intervertebral Disc; Nucleus Pulposus; p38 MAPK; p38 Mitogen-Activated Protein Kinases - genetics; Rats; Signal Transduction
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2019.12.040

The degeneration of intervertebral discs (IVD) is a risk factor for chronic low back pain. Anti-inflammation therapy could alleviate IVD degeneration. IL-10 is an important anti-inflammatory cytokine. However, the effect of IL-10 on IVD has not been fully revealed. The current study is to reveal the effect of IL-10 on IVD and its underlying mechanism. IL-1β was used to induce the degeneration of nucleus pulposus cells (NPCs). mRNA expression level was determined by qPCR. Protein expression level was determined by western blotting. Methylene blue was used to determined the expression of aggrecan. Immunocytochemical staining was used to determined the expression of collagen II. A rat caudal IVD degeneration model was established and used to evaluate the effect of IL-10 on IVD in vivo. IL10 could alleviated NPC degeneration in both morphology and extracellular matrix. IL-10 could increase the mRNA expression of Collagen II, Sox-9, but decrease the mRNA expression of IL-1β, TNFα and Collagen X. IL-10 could also increase the protein level of Collagen II and aggrecan, but decrease that of Collagen X. Western blotting futher revealed the mechanism of the positive effect of IL-10 on IVD. IL-10 reduces phosphorylation level of p38 MAPK effectively. Rat caudal IVD degeneration model futher confirmed the positive effect of IL-10 on IVD degeneration and its mechanism in vivo. The current study demonstrates that exogenous IL-10 treatment can induce an anti-inflammatory response and inhibit p38 MAPK activation to delay IVD degeneration. [Display omitted] •Clearifies the postive effect of exogenous IL-10 on intervertebral disc degeneration (IDD) for the first time.•Explores the mechanism of exogenous IL-10 in treating IDD via inhibiting p38 MAPK activation.•Opens up a new therapeutic approach for clinical treatment of IDD.