Effect of carrageenans on some lipid metabolism components in vitro

• Published in: Carbohydrate polymers 2020-02, Vol.230, p.115629-115629, Article 115629
• Main Authors: Sokolova, E.V., Kravchenko, A.O., Sergeeva, N.V., Davydova, V.N., Bogdanovich, L.N., Yermak, I.M.
• Format: Article
• Language: English
• Subjects: Bile salts; Carrageenans; IL-1β; Lipase; PAMPA; Prostaglandin E2
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2019.115629

•Carrageenans modulate the synthesis of prostaglandin E2.•Carrageenans do not affect lipase activity.•Carrageenans inhibit bile acid permeation through a PAMPA membrane.•Sulfation degree is important for carrageenans ability to inhibit bile salts permeation. The research described here focused on the effect of sulfated red algal polysaccharides (κ-, κ/β-, ι/κ-carrageenan) individually and in combination with lipopolysaccharide (LPS) on the synthesis of prostaglandin E2 (PGE2) and cytokines (interleukin [IL]-1β and IL-6) in whole blood model in vitro. The results demonstrated that, at high concentrations, carrageenans have substantial ability to modulate PGE2 synthesis and stimulate IL-1β and IL-6 synthesis. A low degree of sulfate and high molecular weight were a prerequisite for the ability of carrageenans to modulate PGE2 synthesis. Further, we investigated the ability of the carrageenans alone and in combination with casein to affect bile salt permeability through an artificial membrane imitating the gastrointestinal barrier. The least sulfated κ/β-carrageenan could retain bile salt permeation the most but less efficiently than cholestyramine. The polysaccharides did not affect pancreatic lipase activity. Our data confirm a possible mechanism of the cholesterol-reducing properties of carrageenan.