Dissolution and deacetylation of chitin in ionic liquid tetrabutylammonium hydroxide and its cascade reaction in enzyme treatment for chitin recycling

• Published in: Carbohydrate polymers 2020-02, Vol.230, p.115605-115605, Article 115605
• Main Authors: Ma, Qinyuan, Gao, Xiuzhen, Bi, Xinyu, Han, Qi, Tu, Linna, Yang, Yupeng, Shen, Yanbing, Wang, Min
• Format: Article
• Language: English
• Subjects: Cascade reaction; Chitin; Chitosan; Deacetylation; Dissolution; Ionic liquid
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2019.115605

[Display omitted] •Mild ionic liquids [TBA][OH] was employed on chitin for the first time.•[TBA][OH] showed dissolution and deacetylation abilities on chitin.•Deacetylation of chitin was positively correlated with the solubility of chitin.•A combinational process of [TBA][OH] pretreatment and ReCDA were performed.•The ReCDA activity on chitin was activated with [TBA][OH] pretreatment. Chitin is the second most abundant renewable polymer on earth, and its deacetylated derivative, chitosan, is a highly useful biopolymer. This work studied for the first time the application of ionic liquid (IL) tetrabutylammonium hydroxide ([TBA][OH]) and chitin deacetylase from Rhodococcus equi CGMCC14861 (ReCDA) for the efficient conversion of chitin into chitosan at room temperature. Results confirmed that chitin had good solubility in 18 wt% aqueous [TBA][OH] solution at 80 ℃. In addition, efficient chitin deactylation was observed with high concentrations of [TBA][OH] exceeding 12 wt% and showing potential application in chitin conversion. ReCDA activity on chitin was activated by [TBA][OH] pretreatment. Sequential and simultaneous strategies were also compared, and the results showed that the simultaneous one-pot deacetylation provided the highest acetic acid yield of 3.78 mg/g chitin powder after 24 h. This study serves as a guide for the dissolution and deacetylation of chitin to produce high value-added chitosan products.