Banana MaSPL16 Modulates Carotenoid Biosynthesis during Fruit Ripening through Activating the Transcription of Lycopene β‑Cyclase Genes

Carotenoids are a class of bioactive compounds that exhibit health-promoting properties for humans, but their regulation in bananas during fruit ripening remains largely unclear. Here, we found that the total carotenoid content continued to be elevated along the course of banana ripening and peaked...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2020-02, Vol.68 (5), p.1286-1296
Main Authors: Zhu, Li-sha, Liang, Shu-min, Chen, Lu-lu, Wu, Chao-jie, Wei, Wei, Shan, Wei, Chen, Jian-ye, Lu, Wang-jin, Su, Xin-guo, Kuang, Jian-fei
Format: Article
Language:English
Subjects:
Carotenoids - metabolism
Fruit - enzymology
Fruit - genetics
Fruit - growth & development
Fruit - metabolism
Gene Expression Regulation, Plant
Intramolecular Lyases - genetics
Intramolecular Lyases - metabolism
Musa - enzymology
Musa - genetics
Musa - growth & development
Musa - metabolism
Plant Proteins - genetics
Plant Proteins - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Carotenoids are a class of bioactive compounds that exhibit health-promoting properties for humans, but their regulation in bananas during fruit ripening remains largely unclear. Here, we found that the total carotenoid content continued to be elevated along the course of banana ripening and peaked at the ripening stage followed by a decrease, which is presumably caused by the transcript abundances of carotenoid biosynthetic genes MaLCYB1.1 and MaLCYB1.2. Moreover, a ripening-inducible transcription factor MaSPL16 was characterized, which was a nuclear protein with transactivation activity. Transient transformation of MaSPL16 in banana fruits led to enhanced transcript levels of MaLCYB1.1 and MaLCYB1.2 and hence the total carotenoid accumulation. Importantly, MaSPL16 stimulated the transcription of MaLCYB1.1 and MaLCYB1.2 through directly binding to their promoters. Collectively, our findings indicate that MaSPL16 behaves as an activator to modulate banana carotenoid biosynthesis, which may provide a new target for molecular improvement of the nutritional and bioactive qualities of agricultural crops that accumulate carotenoids.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.9b07134