In syringe hybrid monoliths modified with gold nanoparticles for selective extraction of glutathione in biological fluids prior to its determination by HPLC

• Published in: Talanta (Oxford) 2020-03, Vol.209, p.120566-120566, Article 120566
• Main Authors: Mompó-Roselló, Oscar, Vergara-Barberán, María, Simó-Alfonso, Ernesto F., Herrero-Martínez, José Manuel
• Format: Article
• Language: English
• Subjects: Biological fluids; Glutathione; Gold nanoparticles; Hybrid monolith; Solid-phase extraction
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2019.120566

In this work, a simple device for extraction glutathione (GSH) in biological fluids using a hybrid monolithic material within a polypropylene syringe is developed. For this purpose, glycidyl methacrylate-based monolith was firstly prepared within this housing material, and the polymer was modified with different ligands (ammonia, cysteamine and cystamine). The resulting materials (containing amine or thiol groups, respectively) were then functionalized with gold nanoparticles (AuNPs). The hybrid material that gave the largest AuNPs coverage was selected as solid-phase (SPE) sorbent and several variables affecting the extraction recovery of this compound were investigated. Under optimal conditions, GSH was quantitatively retained at pH 6.0, and then it was desorbed with aqueous dithiothreitol solution and determined, after derivatization with o-phthaldialdehyde, via reversed-phase LC with fluorometric detection. The limit of detection was ca. 1.5 ng mL−1, and the reproducibility between extraction units was below 8% (expressed as relative standard deviation), which demonstrates the robustness of the method. The developed material was also applied for the extraction of GSH in saliva and urine samples yielding recoveries ranging from 86 to 105%. [Display omitted] •Simple in-syringe sorbents based on AuNPs-modified monoliths were prepared.•The hybrid sorbent showed high extraction performance for GSH and good reusability.•The hybrid monolith extraction device provided low LOD in SPE.•The SPE protocol was successfully applied to analyze GSH in human fluids.