A simple fluorescence aptasensor for gastric cancer exosome detection based on branched rolling circle amplification

Exosomes are membrane nanovesicles carrying molecular information that may reflect the biological and genetic characteristics of their parent cells. Numerous studies have demonstrated the potential of exosomes as noninvasive cancer biomarkers. Hence, specific detection of cancer cell-derived exosome...

Full description

Saved in:
Bibliographic Details
Published in:Nanoscale 2020-01, Vol.12 (4), p.2445-2451
Main Authors: Huang, Rongrong, He, Lei, Li, Song, Liu, Hongna, Jin, Lian, Chen, Zhu, Zhao, Yongxiang, Li, Zhiyang, Deng, Yan, He, Nongyue
Format: Article
Language:English
Subjects:
Amplification
Aptamers, Nucleotide - chemistry
Biomarkers
Biosensing Techniques - methods
Biosensors
Cancer
Cell Line, Tumor
Deoxyribonucleic acid
DNA
DNA - chemistry
Exosomes - metabolism
Fluorescence
Fluorescent dyes
Fluorescent Dyes - chemistry
Gastric cancer
Humans
Limit of Detection
Nucleic Acid Amplification Techniques - methods
Organic Chemicals - chemistry
Sensitivity and Specificity
Spectrometry, Fluorescence
Stomach Neoplasms - metabolism
Target detection
Temperature
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Exosomes are membrane nanovesicles carrying molecular information that may reflect the biological and genetic characteristics of their parent cells. Numerous studies have demonstrated the potential of exosomes as noninvasive cancer biomarkers. Hence, specific detection of cancer cell-derived exosomes is of significant importance. Here, we developed a fluorescence assay for the determination of gastric cancer exosomes based on branched rolling circle amplification (BRCA) and an aptamer to target specific exosomes. The designed padlock probe was cyclized after incubation with an aptamer binding with the target exosome. BRCA was triggered by adding a second primer and the resulting long tandem double-stranded DNA product was detected using SYBR Green I as the fluorescent dye. This method demonstrated a high specificity for target exosomes with a detection limit of 4.27 × 10 exosomes per mL. Moreover, plasma from gastric cancer patients was tested to verify the clinical applicability of this assay. Our results demonstrated that this aptamer-based biosensor may show potential for the early diagnosis of gastric cancer.
ISSN:2040-3364
2040-3372
DOI:10.1039/c9nr08747h