Investigation of axonal regeneration of Triturus ivanbureschi by using physiological and proteomic strategies

• Published in: Journal of biosciences 2019-12, Vol.44 (6), p.1-12, Article 145
• Main Authors: Karahisar Turan, Seçil, Onur, Mehmet Ali, Özel Demiralp, Fatma Duygu
• Format: Article
• Language: English
• Subjects: Amphibians; Animals; Aquatic reptiles; Axons - metabolism; Axons - physiology; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cytoskeleton; Damage; Electrophysiology; Elongation; Embryos; Gene Expression Regulation - genetics; Growth factors; Life Sciences; Microbiology; Nerve endings; Nerve Endings - metabolism; Nerve Regeneration - genetics; Nerves; Neurites - metabolism; Peripheral nerves; Plant Sciences; Proteins; Proteomics; Recovery; Regeneration; Regeneration (biological); Reptiles & amphibians; Sciatic nerve; Sciatic Nerve - growth & development; Sciatic Nerve - metabolism; Triturus; Triturus - genetics; Triturus - growth & development; Zoology
• ISSN: 0250-5991; 0973-7138
• DOI: 10.1007/s12038-019-9950-y

Peripheral nerve injuries are frequently observed and successful treatment depends mainly on the injury type, location of the damage, and the elapsed time prior to treatment. The regenerative capacity is limited only to the embryonic period in many mammalian tissues, but urodele amphibians do not lose this feature during adulthood. The main purpose of this study is to define the recovery period after serious sciatic nerve damage of a urodele amphibian, Triturus ivanbureschi . Experimental transection damage was performed on the sciatic nerves of T. ivanbureschi specimens. The recovery period of sciatic nerves were investigated by walking track analysis, electrophysiological recordings, and bottom-up proteomic strategies at different time points during a 35-day period. A total of 34 proteins were identified related to the nerve regeneration process. This study showed that the expression levels of certain proteins differ between distal and proximal nerve endings during the regeneration period. In distal nerve stumps, transport proteins, growth factors, signal, and regulatory molecules are highly expressed, whereas in proximal nerve stumps, neurite elongation proteins, and cytoskeletal proteins are highly expressed.