Acute and sub-acute bisphenol-B exposures adversely affect sperm count and quality in adolescent male mice

• Published in: Chemosphere (Oxford) 2020-03, Vol.242, p.125286-125286, Article 125286
• Main Authors: Ikhlas, Shoeb, Ahmad, Masood
• Format: Article
• Language: English
• Subjects: Age Factors; Animals; Benzhydryl Compounds - toxicity; Bisphenol B; CASA; DNA Damage; Endocrine Disruptors - toxicity; Environmental Pollutants - toxicity; Humans; LD50; Male; Mice; Oxidation-Reduction; Oxidative stress; Oxidative Stress - drug effects; Phenols - toxicity; Sperm Count; Sperm motility; Spermatozoa - drug effects; Spermatozoa - pathology; Toxicity Tests, Acute; Toxicity Tests, Subacute
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2019.125286

Bisphenol-B (BPB), an analogue of bisphenol-A is used in the plastic industry. It has been found to leach from plastic containers leading to its contamination in canned food products. Moreover, it has also been detected in human samples such as sera and urine. BPB is recognized as a potential endocrine disrupting chemical owing to its estrogenic and anti-androgenic nature. Therefore, it was pertinent to study the effect of BPB exposure during the adolescence age (5–6 weeks old) in male mice. Weekly intraperitoneal injections of 5, 10 and 15% LD50 of BPB were given for 2 weeks to acute exposure groups and for 4 weeks to sub-acute exposure groups. BPB exposure induces change in enzymatic and non-enzymatic oxidative stress markers in sperm samples. DNA damage was also observed in sperm cells on acute and sub-acute exposures. Furthermore, BPB exposure led to a marked decline in sperm count and compromised sperm morphology. Computer assisted sperm analysis (CASA) revealed a significant decrease in sperm quality and progressive motility. Thus, both the acute and sub-acute exposures of adolescent male mice to BPB adversely affect the sperms’ quality, functions and morphology. [Display omitted] •BPB induces oxidative stress in spermatozoa of adolescent male mice.•BPB led to DNA damage in sperm cells of the exposed mice.•It also exerted adverse effects on sperm count, and motility.•It adversely affects sperm morphology, and velocity parameters.