Rapid and sensitive detection of diclazuril in chicken samples using a gold nanoparticle-based lateral-flow strip

• Published in: Food chemistry 2020-05, Vol.312, p.126116-126116, Article 126116
• Main Authors: Wang, Zhongxing, Wu, Xiaoling, Liu, Liqiang, Xu, Liguang, Kuang, Hua, Xu, Chuanlai
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal - immunology; Chicken samples; Chickens; Diclazuril; Food safety; Gold; Immunoassay; Limit of Detection; Metal Nanoparticles; Monoclonal antibody; Nitriles - analysis; Time Factors; Triazines - analysis
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2019.126116

•A new hapten of diclazuril was synthesized successfully.•A sensitive and specific monoclonal antibody against diclazuril was developed.•A lateral-flow strip was assembled for detection of diclazuril in chicken samples.•Detection took only 15 min, and the quantitative LOD for diclazuril was 1.08 μg/kg.•The strip assay can be applied in on-site monitoring of chicken samples. In this study, we produced a sensitive and specific monoclonal antibody (mAb) against diclazuril based on a new hapten. The mAb, which belongs to the IgG2 subclass, had a 50%-inhibitory concentration of 0.36 ng/mL. A cross reactivity test revealed that the mAb had good specificity for diclazuril. A gold nanoparticle-based lateral-flow strip was assembled for the rapid screening of diclazuril in raw chicken breast and thigh samples. The strip assay had a visual limit of detection (LOD) of 2 μg/kg and a cut-off value of 20 μg/kg for diclazuril in chicken samples when evaluated with the naked eye. With the aid of a strip scan reader, the proposed assay obtained a quantitative measurement for diclazuril with an LOD of 1.08 μg/kg. Therefore, this gold nanoparticle-based lateral-flow strip assay represents a potentially useful tool for on-site detection and rapid initial screening of diclazuril in chicken samples.