Serum proteins TGFBI, PCSK9, and CCL14 are potential biomarkers for different traditional Chinese medicine syndromes of multidrug‐resistant tuberculosis

Patients with multidrug‐resistant tuberculosis (MDR‐TB) tend to have a long course of anti‐TB treatment and severe side effects. Traditional Chinese Medicine (TCM) has a synergistic effect in attenuation of MDR‐TB. However, the lack of objective biological standards to classify and diagnose MDR‐TB T...

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Published in:Anatomical record (Hoboken, N.J. : 2007) N.J. : 2007), 2020-08, Vol.303 (8), p.2131-2143
Main Authors: Hu, Yu‐Ting, Yi, Wen‐Jing, Jiang, Ting‐Ting, Tu, Hui‐Hui, Wei, Li‐Liang, Shi, Li‐Ying, Liu, Chang‐Ming, Chen, Jing, Han, Yu‐Shuai, Gan, Lin, Li, Zhi‐Bin, Huang, Huai, Li, Ji‐Cheng
Format: Article
Language:English
Subjects:
Adult
Aged
Bioinformatics
Biomarkers
Biomarkers - blood
Chemokines
Chemokines, CC - blood
Chinese medicine
Enzyme-linked immunosorbent assay
Extracellular Matrix Proteins - blood
Female
Humans
Hyperactivity
Immunoglobulins
Kexin
Liquid chromatography
Male
Mass spectroscopy
Medicine, Chinese Traditional
Middle Aged
Multidrug resistant organisms
multidrug‐resistant tuberculosis
Proprotein Convertase 9 - blood
Proprotein convertases
proteomics
Serum proteins
Subtilisin
Tandem Mass Spectrometry
TCM syndromes
Traditional Chinese medicine
Transforming Growth Factor beta - blood
Transforming growth factor-b
Tuberculosis
Tuberculosis, Multidrug-Resistant - blood
Tuberculosis, Multidrug-Resistant - diagnosis
Young Adult
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Summary:Patients with multidrug‐resistant tuberculosis (MDR‐TB) tend to have a long course of anti‐TB treatment and severe side effects. Traditional Chinese Medicine (TCM) has a synergistic effect in attenuation of MDR‐TB. However, the lack of objective biological standards to classify and diagnose MDR‐TB TCM syndromes could result in less effective TCM treatment. Therefore, in this study, we identified differentially expressed proteins (DEPs) in serum of individuals with MDR‐TB TCM syndromes by applying isobaric tags for relative and absolute quantification coupled with two‐dimensional liquid chromatography–tandem mass spectrometry (iTRAQ‐2DLC–MS/MS) method and bioinformatics analysis. The functional analysis of DEPs was also performed. Additionally, DEPs among three different TCM syndromes of MDR‐TB were validated by enzyme‐linked immunosorbent assay (ELISA). Finally, a receiver operating characteristic (ROC) curve was performed to estimate the diagnostic ability of DEPs. A total of 71 DEPs were identified in the three different MDR‐TB TCM syndrome groups such as the pulmonary Yin deficiency (PYD) syndrome group, the Hyperactivity of Fire due to Yin deficiency (HFYD) syndrome group, and the deficiency of Qi and Yin (DQY) syndrome group. The results showed that the expression level of transforming growth factor‐beta‐induced protein ig‐h3 (TGFBI) was lower in the PYD syndrome group (p = .002), the proprotein convertase subtilisin/kexin type 9 (PCSK9) was overexpressed in the HFYD syndrome group (p 
ISSN:1932-8486
1932-8494
DOI:10.1002/ar.24353