LncRNA TUG1 promoted osteogenic differentiation through promoting bFGF ubiquitination

LncRNA TUG1 has the potential to promote the osteogenic differentiation of several cells, but the role of lncRNA TUG1 in osteogenic differentiation of tendon stem/progenitor cells (TSPCs) is still unknown. This study aims to determine the role of lncRNA TUG1 in osteogenic differentiation of TSPCs. b...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2020-01, Vol.56 (1), p.42-48
Main Authors: Yu, Yang, Chen, Ying, Zheng, Yi-jing, Weng, Qi-hao, Zhu, Si-pin, Zhou, Dong-sheng
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biomedical and Life Sciences
Biomedical materials
Cbfa-1 protein
Cell Biology
Cell Culture
Cell differentiation
CELL GROWTH/DIFFERENTIATION/APOPTOSIS
Cells (biology)
Developmental Biology
Differentiation (biology)
Fibroblast growth factor 2
Gene expression
Immunoprecipitation
Life Sciences
Progenitor cells
Protein expression
Proteins
Ribonucleic acid
RNA
Stem Cells
Ubiquitination
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Summary:LncRNA TUG1 has the potential to promote the osteogenic differentiation of several cells, but the role of lncRNA TUG1 in osteogenic differentiation of tendon stem/progenitor cells (TSPCs) is still unknown. This study aims to determine the role of lncRNA TUG1 in osteogenic differentiation of TSPCs. bFGF, RUNX2, and Osterix protein expressions were detected by western blot. LncRNA TUG1 and bFGF expression was detected by qRT-PCR. RNA immunoprecipitation (RIP) assay was used to confirm the interaction between TUG1 and bFGF2. Ubiquitination assay was used to determine the ubiquitination of bFGF protein. During osteogenic differentiation, the protein expression of bFGF was significantly downregulated in TSPCs, and the expression of TUG1 was significantly elevated in TSPCs. Interfering TUG1 or overexpressing bFGF suppressed osteogenic differentiation of TSPCs. In addition, lncRNA TUG1 interacted with bFGF, and lncRNA TUGl promoted the ubiquitination of bFGF protein. We also determined that lncRNA TUG1 downregulated bFGF protein expression through promoting the ubiquitination of bFGF. LncRNA TUGl promoted the osteogenic differentiation of TSPCs through promoting bFGF ubiquitination.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-019-00410-y