An adhesive and injectable nanocomposite hydrogel of thiolated gelatin/gelatin methacrylate/Laponite® as a potential surgical sealant

[Display omitted] Hemostatic adhesive hydrogels as sealants for surgical operations are one of the focus of the researches in the field of injectable materials. Herein, we evaluated the potential application of a mechanically robust nanocomposite hydrogel with significant adhesion strength and short...

Full description

Saved in:
Bibliographic Details
Published in:Journal of colloid and interface science 2020-03, Vol.564, p.155-169
Main Authors: Rajabi, Negar, Kharaziha, Mahshid, Emadi, Rahmatollah, Zarrabi, Ali, Mokhtari, Hamidreza, Salehi, Sahar
Format: Article
Language:English
Subjects:
Methacrylate gelatin
Surgical sealants
Thiol-methacrylate chemistry
Thiolated gelatin
Visible photo crosslinking
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] Hemostatic adhesive hydrogels as sealants for surgical operations are one of the focus of the researches in the field of injectable materials. Herein, we evaluated the potential application of a mechanically robust nanocomposite hydrogel with significant adhesion strength and shorter blood clotting time. This hydrogel was composed of thiolated gelatin (Gel-SH) and gelatin methacrylate (GelMA) as the main matrix to support cell viability and proliferation, while polydopamine functionalized Laponite® (PD-LAP) were introduced to the structure to improve the mechanical properties, adhesion strength, and blood clotting. This hydrogel formed via Michael reaction between Gel-SH and GelMA, and covalent interaction between PD-LAP and hydrogel. Results revealed that presence of PD-LAP significantly controlled the swelling ratio, biodegradability, and mechanical properties of nanocomposite hydrogels. Tensile and compressive strength of nanocomposite hydrogels were measured in the range of 22–84 kPa and 54–153 kPa, respectively. Furthermore, nanocomposite hydrogels revealed excellent recovery ability, strong tissue adhesiveness and significantly less blood clotting time than Gel-SH/GelMA hydrogel (2.25 min). In the culture with L929 fibroblasts cells, viability more than 97% and high proliferation after 5 days of culture was estimated. The simplicity, low-cost, tunable mechanical properties, short blood clotting time, and cytocompatibility of the hydrogels composed of Gel-SH, GelMA, and PD-LAP highlight its potential as hemostat sealants.
ISSN:0021-9797
1095-7103
DOI:10.1016/j.jcis.2019.12.048