Characterization of a group of peptides for potential applications in hydrogen phosphate and heavy metals accumulation

Phosphorus and heavy metals are discarded to the domestic sewage in our daily life, it is necessary to find easy methods for phosphorus and heavy metals accumulation. Here, a group of short peptides (ChBpHs) were found to react with hydrogen phosphate forming insoluble substances. ChBpHs are compose...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2020-05, Vol.246, p.125735-125735, Article 125735
Main Authors: Zhang, Zhikun, Zhou, Yingshun, Wang, Ying, Zhang, Luhua, Li, Ying, Wang, Guangxi, Ji, Hongsheng, Liu, Yao
Format: Article
Language:English
Subjects:
ChBpHs
Escherichia coli
Histidine
Hydrogen
Ions
Metals, Heavy - chemistry
Nickel
Peptides
Peptides - chemistry
Phosphate affinity
Phosphates - chemistry
Phosphorus
Sewage - chemistry
Waste Disposal, Fluid - methods
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Summary:Phosphorus and heavy metals are discarded to the domestic sewage in our daily life, it is necessary to find easy methods for phosphorus and heavy metals accumulation. Here, a group of short peptides (ChBpHs) were found to react with hydrogen phosphate forming insoluble substances. ChBpHs are composed by a choline binding peptides (ChBp) and a C-terminal histidine rich tail. The reaction region to hydrogen phosphate was determined at 1-18th amino acid in ChBp. The affinities of ChBpHs are different, with minimum react concentrations of Na2HPO4 ranging from 2 to 12 mM. In addition, the C-terminal histidine tail enables ChBpHs with affinities to metal ions in vitro. Prokaryotic expression of ChBpH1 in Escherichia coli resulted in the reduction of soluble hydrogen phosphate in the culture medium. The accumulation of phosphate is time and concentration dependent, maximum reduction was detected at 24 h post induction (23% in phosphate rich medium and 14% in normal medium). The reduction of nickel ions (about 20%) was only detected after cells were broken. In conclusion, this preliminary investigation of ChBpHs indicates the potential applications for bioconcentration of soluble phosphate in the future. •Identified a group of polypeptides (ChBpHs) with reaction to hydrogen phosphate.•The minimum react concentrations of Na2HPO4 down to 2 mM.•ChBpH1 increased the bioconcentration of phosphate in E.coli.
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2019.125735