Immediate and residual antifungal activity of compounds used for whole body and adjuvant topical therapy against Microsporum canis: an in vitro study

• Published in: Veterinary dermatology 2020-08, Vol.31 (4), p.272-e64
• Main Author: Moriello, Karen A.
• Format: Article
• Language: English
• Subjects: Administration, Topical; Animals; Antifungal activity; Antifungal Agents - therapeutic use; Cat Diseases - drug therapy; Cats; Chlorhexidine; Clotrimazole; Enilconazole; Hair; Hair - microbiology; Immunoglobulin E; Ketoconazole; Miconazole; Microbial Sensitivity Tests; Microsporum - drug effects; Microsporum canis; Shampoos; Spores; Sulfur; Terbinafine; Tinea - drug therapy; Tinea - veterinary; Toothbrushes
• ISSN: 0959-4493; 1365-3164
• DOI: 10.1111/vde.12842

Background Topical antifungal therapy is recommended to disinfect hairs of dermatophyte‐infected animals. Objective To determine the immediate and residual (24, 48 and 72 h) antifungal activity of commonly used products for focal (n = 11) and whole body application (n = 3). Animals Hair samples from naturally infected kittens. Methods and materials Immediate antifungal activity was assessed using isolated infected spores. In a more robust challenge, toothbrushes containing whole infected hairs were repeatedly treated with products until culture‐negative. Residual activity was determined by treating 0.04 g of uninfected hair with test products, allowing hairs to dry and then plating hairs onto fungal culture plates inoculated with Microsporum canis. Residual activity was assessed at 24, 48 and 72 h post‐treatment. Results All products showed good efficacy against isolated infected spores. Two products required three treatments to disinfect whole infected hairs (miconazole 0.2% and ketoconazole 0.15%); all other products were efficacious after one treatment. For each product residual activity at 24, 48 and 72 h was similar. For the whole body products, lime sulfur and enilconazole showed residual activity, but as expected none was detected for 2% miconazole nitrate/ 2% chlorhexidine shampoo. For focal therapy products, residual activity was detected for clotrimazole 1%, terbinafine 1%, miconazole at 0.2, 1 or 2% (with or without chlorhexidine), climbazole 0.5% with chlorhexidine gluconate, and ketoconazole 1% with chlorhexidine gluconate. A ketoconazole 0.15% with 0.15% chlorhexidine gluconate showed no residual activity. Conclusions and clinical importance Findings support twice weekly application of enilconazole and lime sulfur, and application of adjuvant focal topical therapy daily or every other day. Résumé Contexte Le traitement topique antifongique est recommandé pour désinfecter les poils infectés par les dermatophytes chez l'animal. Objectif Déterminer l'activité antifongique résiduelle et immédiate (24, 48 et 72h) des produits fréquemment utilisés pour application focale (n = 11) et sur tout le corps (n = 3). Sujets Poils prélevés sur chatons naturellement infectés. Matériels et méthodes L'activité antifongique immédiate a été déterminée à l'aide de spores infectant. Dans un objectif plus poussé, des brosses à dent contenant des poils infectés ont été traités plusieurs fois jusqu’à culture négative. L'activité résiduelle a été déterminée par traite