New Constituents from the Low Polar Fraction of the Fruits of Crataegus dahurica and Their Anti‐Inflammatory Activity in RAW264.7 Cells

• Published in: Chemistry & biodiversity 2020-02, Vol.17 (2), p.e1900609-n/a
• Main Authors: Wang, Bo‐Bo, Gao, Yang, Chen, Li‐Ya, Zhang, Chang‐Long, Zhang, Xiao‐Qing, Zhang, Hai‐Long
• Format: Article
• Language: English
• Subjects: active constituents; Animals; anti-inflammatory activity; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; Biphenyl; Biphenyl Compounds - chemistry; Biphenyl Compounds - isolation & purification; Biphenyl Compounds - pharmacology; Cell Survival - drug effects; Constituents; Crataegus; Crataegus - chemistry; Crataegus - metabolism; Crataegus dahurica; Cytotoxicity; Dyspepsia; edible herb; Fruit - chemistry; Fruit - metabolism; Fruits; Inflammation; Lipopolysaccharides - toxicity; Macrophages - cytology; Macrophages - drug effects; Macrophages - metabolism; Magnetic Resonance Spectroscopy; Mice; Molecular Conformation; new biphenyl derivatives; Nitric Oxide - metabolism; NMR; Nuclear magnetic resonance; Petroleum ether; RAW 264.7 Cells; Spectrometry; Toxicity
• ISSN: 1612-1872; 1612-1880
• DOI: 10.1002/cbdv.201900609

The fruit of Crataegus dahurica Koehne was used to treat the disease of infantile indigestion and dyspepsia as an ethnic medicine and food. As a continuous work on finding the active constituents from the edible herbs, four new biphenyl derivatives (1–4), together with two known compounds (5 and 6), were obtained from the petroleum ether fraction of the fruits of C. dahurica. Their structures were determined by the extensive 1D and 2D NMR spectra and HR‐MS spectrometry. Furthermore, the anti‐inflammatory activities of all the isolated compounds were investigated, in which compound 4 showed moderately inhibitory effects on NO production in RAW264.7 cells without inducing cytotoxicity.