Structural insights reveal the effective Spirulina platensis cell wall dissociation methods for multi-output recovery

• Published in: Bioresource technology 2020-03, Vol.300, p.122628-122628, Article 122628
• Main Authors: Chen, Wangsun, Xu, Jingliang, Yu, Qiang, Yuan, Zhenhong, Kong, Xiaoying, Sun, Yongming, Wang, Zhongming, Zhuang, Xinshu, Zhang, Yu, Guo, Ying
• Format: Article
• Language: English
• Subjects: Cell Wall; Cell wall dissociation mechanism; Ions; Microalgae; Multi-output recovery; Spirulina; Ultracellular structure
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2019.122628

•Uncover structural insights into cell wall dissociation.•Develop the prospect of synthesized multi-output recovery using an integrated strategy.•Propose Spirulina platensis cell wall dissociation mechanisms: attacking by OH− or H+. In this work, Spirulina platensis cells harvested in the exponential and equilibrium phases with intact and broken cell walls were treated through a set of alkaline or acidic conditions including alkalis and acids, with solutions of pH 0.0–14.0. The effective Spirulina platensis cell wall dissociation methods for multi-output recovery were obtained. SEM and FTIR were applied to characterize the alkaline and acid treatment details, and Spirulina platensis cell wall dissociation mechanisms, via attacks by OH− or H+, were then proposed. Overall, this study highlights the synthesized multi-output algal product in an integrated strategy with ultracellular structural insight and is valuable for understanding the specific roles of attack ions.