Melatonin improves development, mitochondrial function and promotes the meiotic resumption of sheep oocytes from in vitro grown secondary follicles

The aim of this study was to evaluate follicular survival and development of ovine isolated secondary follicles cultured in medium containing fixed or sequential concentrations of melatonin and further oocyte maturation. Isolated secondary follicles were cultured for 18 days in α-MEM+ alone (control...

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Bibliographic Details
Published in:Theriogenology 2020-03, Vol.144, p.67-73
Main Authors: Barros, V.R.P., Monte, A.P.O., Santos, J.M.S., Lins, T.L.B.G., Cavalcante, A.Y.P., Gouveia, B.B., Müller, M.C., Oliveira, J.L., Donfack, N.J., Araújo, V.R., Matos, M.H.T.
Format: Article
Language:English
Subjects:
Meiosis
Mitochondrial function
Ovine
Pineal hormone
ROS
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Summary:The aim of this study was to evaluate follicular survival and development of ovine isolated secondary follicles cultured in medium containing fixed or sequential concentrations of melatonin and further oocyte maturation. Isolated secondary follicles were cultured for 18 days in α-MEM+ alone (control) or with different concentrations of melatonin (100, 500 or 1000 pg/mL) or sequential concentrations of melatonin (Mel Seq: Day 6 = 100; Day 12 = 500; Day 18 = 1000 pg/mL). The percentages of morphologically normal follicles and antral cavity formation increased significantly in 1000 pg/mL melatonin compared to the other treatments. After 18 days, 1000 pg/mL melatonin (Mel 100) showed a greater (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2019.12.006