Enhanced selective enrichment of partial nitritation and anammox bacteria in a novel two-stage continuous flow system using flat-type poly (vinylalcohol) cryogel films

[Display omitted] •Stable PN with high AOR 1.68 ± 0.12 kg N m−3 d−1 in short acclimation of 8 days.•Flat-type PVAcryogel elevated endurance of immobilized anammox bacteria to O2 shock.•A novel two-stage PN-anammox system achieved 90% N removal at short HRT of 7.7 h.•Microbial analysis confirmed enha...

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Bibliographic Details
Published in:Bioresource technology 2020-03, Vol.300, p.122546-122546, Article 122546
Main Authors: Choi, Minkyu, Chaudhary, Ramjee, Lee, Minjoo, Kim, Jihyun, Cho, Kyungjin, Chung, Yun-Chul, Bae, Hyokwan, Park, Joonhong
Format: Article
Language:English
Subjects:
Ammonium Compounds
Anammox
Bacteria
Bioreactors
Cryogels
Immobilization
Nitrogen
Oxidation-Reduction
Partial nitritation
PVA cryogel
Reactor microbiome
Waste Water
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Summary:[Display omitted] •Stable PN with high AOR 1.68 ± 0.12 kg N m−3 d−1 in short acclimation of 8 days.•Flat-type PVAcryogel elevated endurance of immobilized anammox bacteria to O2 shock.•A novel two-stage PN-anammox system achieved 90% N removal at short HRT of 7.7 h.•Microbial analysis confirmed enhanced selective growth of AOB and anammox bacteria. To improve stability of nitrogen removal in partial nitritation (PN)-anammox process, flat-type cryogel films using poly (vinylalcohol) named as FT-CPVAF were applied in continuous reactors. Stable PN operation was maintained with short acclimation of 8 days and ammonium oxidation rate of 1.68 ± 0.12 kg N m−3 d−1 comparatively higher than previous studies. The nitrogen removal, initially inhibited by an oxygen shock, was immediately reactivated with short lag-period by immobilization of anammox bacteria in FT-CPVAF. A novel two-stage PN-anammox process was operated in a continuous flow using FT-CPVAF for treatment of ammonium-rich synthetic wastewater (influent 315 mg NH4+-N L−1) showing 89.6 ± 0.76% of nitrogen removal at short hydraulic retention time (7.7 h). The use of FT-CPVAF enhanced selective enrichment of AOB and anammox bacter ia confirmed by high-throughput sequencing of i.e., relative abundances of Nitrosomonas europaea C-31 (37.14% in PN reactor) and ‘Candidatus Jettenia caeni’ (34.36% in anammox reactor).
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2019.122546