Analysis of candidate genes expression associated with defense responses to root and collar rot disease caused by Phytophthora capsici in peppers Capsicum annuum

• Published in: Genomics (San Diego, Calif.) Calif.), 2020-05, Vol.112 (3), p.2309-2317
• Main Authors: Bagheri, Leila Mohammad, Nasr-Esfahani, Mehdi, Abdossi, Vahid, Naderi, Davood
• Format: Article
• Language: English
• Subjects: AP2/ERF; Capsicum - anatomy & histology; Capsicum - genetics; Capsicum - growth & development; cDNA; Disease Resistance - genetics; MIP gene; Phenotype; Phylogeny; Phytophthora - classification; Phytophthora - genetics; Phytophthora - isolation & purification; Phytophthora - metabolism; Plant Diseases - etiology; Plant Diseases - genetics; Primers; Proteinase; Q-RTPCR; Real-Time Polymerase Chain Reaction; XLOC
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2020.01.002

Root and collar rot disease caused by Phytophthora capsici (Leonian) is one of the most serious diseases in pepper, Capsicum annuum L. Knowledge about resistant genes is limited in pepper accessions to P. capsici. In this study, a diverse collection of 37 commercial edible and ornamental genotypes, and implication of seven novel candidate DEGs genes (XLOC_ 021757, XLOC_021821, XLOC_012788, XLOC_011295, XLOC_021928, XLOC_015473 and XLOC_000341) were up-regulated on resistant and susceptible pepper cultivars, through real-time polymerase chain reaction (qPCR) at transplanting and maturing stages. All seven related defense-gene candidates were up-regulated in all inoculated accessions to P. capsici, but these genes were highly expressed in resistant ones, 19OrnP-PBI, 37ChillP-Paleo, and 23CherryP-Orsh. The transcriptional levels of the seven related candidate DEGs were 5.90, 5.64, 5.62, 5.18, 3.94, 3.69, 3.16 folds higher in the resistant pepper genotypes, than the control ones, non-inoculated genotypes respectively. The candidate genes expressed herein, will provide a basis for further gene cloning and functional verification studies, and also will aid in an understanding of the regulatory mechanism of pepper resistance to P. capsici. •DEGs of XLOC group, are likely to be defense-related genes to P. capsici infection•and showed to what extend play important roles in combating the fungal pathogen, P. capsici,•As was in pepper cv. “19OrnP-PBI”, which is regarded as one of the most resistant pepper over here.•These studies may be contributing to decrease the devastating effects of Phytophthora species,•On Solanaceae and other related family, through the producing program for resistant pepper varieties.