Quantitative analysis of metabolites in glucose metabolism in the aqueous humor of patients with central retinal vein occlusion

• Published in: Experimental eye research 2020-02, Vol.191, p.107919-107919, Article 107919
• Main Authors: Wei, Pinghui, He, Meiqin, Teng, He, Han, Guoge
• Format: Article
• Language: English
• Subjects: Aqueous humor (AH); Central retinal vein occlusion (CRVO); Glucose metabolism; Retinal metabolism
• ISSN: 0014-4835; 1096-0007
• DOI: 10.1016/j.exer.2020.107919

Quantitative analysis of aqueous humor (AH) was performed to investigate glucose metabolism in patients with central retinal vein occlusion (CRVO), and to explore metabolic changes after anti-vascular endothelial growth factor (VEGF) treatment. AH samples were collected from 35 patients. Participants diagnosed with CRVO (n = 15) were compared to participants who underwent cataract surgery (n = 20). Thirteen of the participants with CRVO received second-round anti-VEGF treatments. Ultra-high performance liquid chromatography tandem-mass spectrometry (UHPLC-MS/MS) was used to quantify metabolites of the AH. Central macular thickness (CMT) and retinal ganglion cell layer (RGC) thickness were measured using spectral-domain optical coherence tomography. Thirteen metabolites involved in glucose metabolism were identified. Among these metabolites, succinate, glutamate, and glutamine were significantly decreased for the CRVO group (p = 0.028, 0.009, and 0.017, respectively). The α-ketoglutarate/citrate (K/C) ratio had a significant positive correlation with glutamine levels for both control (r = 0.922, p