Catfish lymphocytes expressing CC41-reactive leukocyte immune-type receptors (LITRs) proliferate in response to Edwardsiella ictaluri infection in vitro

Monoclonal antibodies (mAbs) CC34 and CC41 recognize overlapping subsets of leukocyte immune-type receptors (LITRs). The mAb CC34 was raised against the clonal TS32.15 cytotoxic T cell line and the mAb CC41 was raised against the clonal NK cell line TS10.1. In this study, an in vitro model was devel...

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Bibliographic Details
Published in:Developmental and comparative immunology 2020-05, Vol.106, p.103610-103610, Article 103610
Main Authors: Blackmon, Laura E., Quiniou, Sylvie M.A., Wilson, Melanie, Bengtén, Eva
Format: Article
Language:English
Subjects:
Biotechnology
Catfish
Cell culture
Cell-mediated immunity
coculture
Cytotoxic cells
Cytotoxicity
dyes
Edwardsiella ictaluri
Flow cytometry
In vitro infection model
Infections
Kidneys
Leukocyte immune-type receptors (LITRs)
Leukocytes
Lymphocytes
Lymphocytes T
Macrophages
Monoclonal antibodies
Natural killer cells
Peripheral blood
Receptors
staining
T-lymphocytes
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Summary:Monoclonal antibodies (mAbs) CC34 and CC41 recognize overlapping subsets of leukocyte immune-type receptors (LITRs). The mAb CC34 was raised against the clonal TS32.15 cytotoxic T cell line and the mAb CC41 was raised against the clonal NK cell line TS10.1. In this study, an in vitro model was developed to monitor CC34- and CC41-reactive cells in response to Edwardsiella ictaluri infection. Briefly, head kidney leukocytes and peripheral blood lymphocytes (PBL) were isolated from individual catfish and labeled with CellTrace Violet and CellTrace FarRed dye, respectively. Head kidney-derived macrophages were infected with E. ictaluri and then cocultured with autologous PBL. The combined cell cultures were then analyzed using flow cytometry. A significant increase in CC41 staining was observed in the PBL population at 2, 5 and 7 days after culture, which suggest that LITRs are involved in cell-mediated immunity to E. ictaluri. •Development of an assay to measure cellular responses against intracellular infection.•Cell labeling monitors distinct populations in autologous mixed leukocyte reactions.•E. ictaluri infected head kidney macrophages stimulate LITR expressing lymphocytes.•CC41+ lymphocytes proliferate in response to E. ictaluri infected macrophages.
ISSN:0145-305X
1879-0089
DOI:10.1016/j.dci.2020.103610