Genetic and Phenotypic Characteristics of a Multi-strain Probiotic for Broilers

Bacteria isolated from different segments of the gastro-intestinal tract (GIT) of healthy free-range broilers were screened for probiotic properties. Six strains were selected and identified as Lactobacillus gallinarum , Lactobacillus johnsonii , Lactobacillus salivarius , Lactobacillus crispatus ,...

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Bibliographic Details
Published in:Current microbiology 2020-03, Vol.77 (3), p.369-387
Main Authors: Neveling, Deon P., Ahire, Jayesh J., Laubscher, Wikus, Rautenbach, Marina, Dicks, Leon M. T.
Format: Article
Language:English
Subjects:
Abnormalities
Bacillus amyloliquefaciens
Bile
Biofilms
Biomedical and Life Sciences
Biotechnology
Enterococcus faecalis
Exopolysaccharides
Gastrointestinal system
Hydrogen peroxide
Hydrolase
Intestine
Lactobacillus crispatus
Lactobacillus gallinarum
Lactobacillus johnsonii
Lactobacillus salivarius
Life Sciences
Microbiology
Phytase
Probiotics
RecA protein
rRNA 16S
Strains (organisms)
Surfactin
Yogurt
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Summary:Bacteria isolated from different segments of the gastro-intestinal tract (GIT) of healthy free-range broilers were screened for probiotic properties. Six strains were selected and identified as Lactobacillus gallinarum , Lactobacillus johnsonii , Lactobacillus salivarius , Lactobacillus crispatus , Enterococcus faecalis and Bacillus amyloliquefaciens based on 16S rRNA, gyrB and recA gene sequence analyses. All six strains produced exopolysaccharides (EPS) and formed biofilms under conditions simulating the broiler GIT. Lactobacillus johnsonii DPN184 and L. salivarius DPN181 produced hydrogen peroxide, and L. crispatus DPN167 and E. faecalis DPN94 produced bile salt hydrolase (BSH) and phytase. Bacillus amyloliquefaciens DPN123 produced phytase, amylase, surfactin and iturin A1. No abnormalities were observed when broilers were fed the multi-strain combination, suggesting that it could be used as a probiotic.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-019-01797-3