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A sensitive amperometric detection of neurotransmitter acetylcholine using carbon dot‐modified carbon paste electrode

Acetylcholine is a neurotransmitter, which is located at the intersections of the nerve and muscles in the lymph nodes of the internal organs motor systems and in various parts of the central nervous system. A decrease of acetylcholine in brain is associated with Alzheimer‘s disease. That is why it...

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Bibliographic Details
Published in:Biotechnology and applied biochemistry 2021-02, Vol.68 (1), p.20-29
Main Authors: Bodur, Onur Can, Dinç, Saliha, Özmen, Mustafa, Arslan, Fatma
Format: Article
Language:English
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Summary:Acetylcholine is a neurotransmitter, which is located at the intersections of the nerve and muscles in the lymph nodes of the internal organs motor systems and in various parts of the central nervous system. A decrease of acetylcholine in brain is associated with Alzheimer‘s disease. That is why it is an important agent for this disease. In this study, a bienzymatic biosensor system with acetylcholine esterase and choline oxidase was prepared with carbon paste electrode modified with carbon nano Dot‐(3‐Aminopropyl) triethoxysilane (CDs‐APTES) for determination of the amount of acetylcholine. Acetylcholine esterase and choline oxidase enzymes were immobilized onto a modified carbon paste electrode by cross‐linking with glutaraldehyde. Determination of acetylcholine was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V versus Ag/AgCl. The effect of temperature, pH, and substrate concentration on the acetylcholine response of the prepared biosensor was investigated. In addition, the optimum CDs‐APTES amount, the linear operating range of the biosensor, and the interference effect were also investigated. A Sensitive Amperometric Detection of Neurotransmitter Acetylcholine Using Carbon Dot‐Modified Carbon Paste Electrode
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.1886