Effects of ganciclovir treatment in a murine model of cytomegalovirus‐induced hearing loss

Objective To determine whether ganciclovir (GCV) treatment reduces sensorineural hearing loss in cytomegalovirus (CMV)‐infected mice. The effects of GCV on viral load, absolute neutrophil count (ANC), and outer hair cell (OHC) integrity were also investigated. Methods Infected BALB/c mice were inocu...

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Bibliographic Details
Published in:The Laryngoscope 2020-04, Vol.130 (4), p.1064-1069
Main Authors: Haller, Travis J., Price, Melissa S., Lindsay, Spencer R., Hillas, Elaine, Seipp, Michael, Firpo, Matthew A., Park, Albert H.
Format: Article
Language:English
Subjects:
Antiviral drugs
Cytomegalovirus
ganciclovir
Hearing loss
mouse
valganciclovir
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Summary:Objective To determine whether ganciclovir (GCV) treatment reduces sensorineural hearing loss in cytomegalovirus (CMV)‐infected mice. The effects of GCV on viral load, absolute neutrophil count (ANC), and outer hair cell (OHC) integrity were also investigated. Methods Infected BALB/c mice were inoculated with murine CMV on postnatal day 3. Those treated with GCV received an intraperitoneal injection twice a day for 14 days. Auditory thresholds were assessed using distortion product otoacoustic emission (DPOAE) and auditory brainstem response (ABR) testing 4 weeks after inoculation. Temporal bones were used for determination of viral load by quantitative polymerase chain reaction and hair cell quantification by scanning electron microscopy. ANCs were completed by an automated hematology analyzer, with manual review for confirmation. Results GCV‐treated CMV‐infected mice had lower ABR (P
ISSN:0023-852X
1531-4995
DOI:10.1002/lary.28134