Plasma extracellular microRNAs are related to AIDS/cerebral toxoplasmosis co‐infection

This study investigated the potential of five miRNA candidates for cerebral toxoplasmosis/HIV co‐infection (CT/HIV) biomarkers. miR‐155‐5p, miR‐146a‐5p, miR‐21‐5p, miR‐125b‐5p and miR‐29c‐3p were tested in 79 plasma divided into groups: 32 CT/HIV patients; 27 individuals with asymptomatic toxoplasmo...

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Bibliographic Details
Published in:Parasite immunology 2020-04, Vol.42 (4), p.e12696-n/a
Main Authors: Pereira, Ingrid de Siqueira, Maia, Marta Marques, Cruz, Allecineia Bispo, Telles, João Paulo Marochi, Vidal, Jose Ernesto, Gava, Ricardo, Meira‐Strejevitch, Cristina Silva, Pereira‐Chioccola, Vera Lucia
Format: Article
Language:English
Subjects:
Blood cells
cerebral toxoplasmosis
cytokine regulation
Diagnosis
extracellular miRNA
Gene expression
HIV
Human immunodeficiency virus
Kruskal-Wallis test
MicroRNAs
miRNA
Peripheral blood
Plasma
Statistical analysis
Toxoplasmosis
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Summary:This study investigated the potential of five miRNA candidates for cerebral toxoplasmosis/HIV co‐infection (CT/HIV) biomarkers. miR‐155‐5p, miR‐146a‐5p, miR‐21‐5p, miR‐125b‐5p and miR‐29c‐3p were tested in 79 plasma divided into groups: 32 CT/HIV patients; 27 individuals with asymptomatic toxoplasmosis (AT); and 20 individuals seronegative for toxoplasmosis (NC). From each was collected peripheral blood/EDTA for laboratory diagnosis. Blood cells for DNA extractions (molecular diagnosis), plasma for RNA extractions (gene expression) and ELISA (serological diagnosis). miRNA expression was performed by qPCR, and values were expressed in Relative Quantification (RQ). Among the five miRNAs, miR‐21‐5p and miR‐146a‐5p were up‐expressed in CT/HIV group when compared with AT and NC groups. RQ means for miR‐21‐5p and miR‐146a‐5p in CT/HIV group were 3.829 and 2.500, while in AT group, were 1.815 and 1.661, respectively. Differences between 3 groups were statistically significant (Kruskal‐Wallis ANOVA test), as well as CT/HIV and AT groups (Mann‐Whitney test). Plasma of CT/HIV and AT groups expressed similar levels of miR‐29c‐3p, miR‐155‐5p and miR‐125b‐5p. As NC group was different of CT/HIV and AT groups, differences between three groups were statistically significant (Kruskal‐Wallis ANOVA test). No difference was shown between CT/HIV and AT groups (Mann‐Whitney test). These results suggest the host miRNAs modulation by Toxoplasma gondii.
ISSN:0141-9838
1365-3024
DOI:10.1111/pim.12696