A high-sensitive sensor with HEPES-enhanced electrochemiluminescence of benzo[3]uril for Fe3+ and its application in human serum

An electrochemiluminescence (ECL) sensor based on a benzo[3]uril-modified glassy carbon electrode with sensitized luminescence, with the coexistence of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) as the coreactant, was successfully constructed. The sensitization mechanism was proposed...

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Bibliographic Details
Published in:Analyst (London) 2020-03, Vol.145 (5), p.1810-1816
Main Authors: Yao, Lei, Qiu, Fei, Xian-Yi, Jin, Jian-Mei, Yang, Liu, Mao, Qing-Mei Ge, Cong, Hang, Zhu, Tao
Format: Article
Language:English
Subjects:
Aqueous solutions
Electrochemiluminescence
Glassy carbon
Luminescence
Selectivity
Sensors
Online Access:Get full text
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Summary:An electrochemiluminescence (ECL) sensor based on a benzo[3]uril-modified glassy carbon electrode with sensitized luminescence, with the coexistence of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) as the coreactant, was successfully constructed. The sensitization mechanism was proposed by analyzing the results of the control experiments for establishing the relationship of the luminescence effect with the concentration of HEPES. Under the optimized conditions, the fabricated sensor system was applied for the detection of Fe3+ in an aqueous solution with good sensitivity and selectivity. A low detection limit of 0.41 nM was achieved, indicating superior sensor performance over the previous analytical methods. The ECL sensor system was employed for the detection of Fe3+ in human serum samples to produce excellent recoveries ranging from 96.17% to 101.81%.
ISSN:0003-2654
1364-5528
DOI:10.1039/c9an02156f