An investigation on the prevalence of peste des petits ruminants in the camels of Sindh, Pakistan

The present study investigated the status of peste des petits ruminants (PPR) for the first time in the camels of Pakistan. The samples were collected from the camel residing area of Sindh, Pakistan, and analyzed for breeds (Dhatti and Larri), districts (Tharparkar and Umerkot), age (young, adult, a...

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Bibliographic Details
Published in:Tropical animal health and production 2020-07, Vol.52 (4), p.1863-1867
Main Authors: Rahman, Hamidur, Kamboh, Asghar Ali, Abro, Shahid Hussain, Abubakar, Muhammad
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigens
Biomedical and Life Sciences
Camelidae
Camels
Camelus
Confidence intervals
Disease control
Disease resistance
Enzyme-Linked Immunosorbent Assay
Enzymes
Feces
Feces - virology
Female
Life Sciences
Male
Pakistan - epidemiology
Peste des petits ruminants
Peste-des-Petits-Ruminants - epidemiology
Peste-des-petits-ruminants virus - isolation & purification
Polymerase chain reaction
Prevalence
Regular Articles
RNA-directed DNA polymerase
Seroepidemiologic Studies
Serology
Veterinary Medicine/Veterinary Science
Viruses
Zoology
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Summary:The present study investigated the status of peste des petits ruminants (PPR) for the first time in the camels of Pakistan. The samples were collected from the camel residing area of Sindh, Pakistan, and analyzed for breeds (Dhatti and Larri), districts (Tharparkar and Umerkot), age (young, adult, and old), and sexes (male and female). The sera samples ( n  = 200) were analyzed for the detection of antibodies using a competitive enzyme-linked immunosorbent assay (cELISA). Moreover, the nasal and fecal samples were screened for the PPR virus. Finally, the positive nasal and fecal samples were validated using reverse transcriptase-polymerase chain reaction (RT-PCR) and immunocapture enzyme-linked immunosorbent assay (Ic-ELISA). The cELISA results showed an overall prevalence of 8.5% PPR in the study area. The camels of Tharparkar (10.9%; 95% confidence interval (CI) 9.2–12.9) showed higher seroprevalence of PPR antibodies than those of Umerkot (5.5%; 95% CI 4.1–7.2). Moreover, the Larri breed exhibited slightly greater resistance against the disease, because the camels of Dhatti breed (9.0%; 95% CI 7.5–11.0) exhibited a numerically higher ( p >  0.05) seroprevalence of PPR in comparison with those of Larri breed (7.9%; 95% CI 6.4–9.9). Furthermore, the young and old camels were more susceptible to the disease attack, because the adults (6.3%; 95% CI 5.0–7.8) exhibited significantly ( p  
ISSN:0049-4747
1573-7438
DOI:10.1007/s11250-020-02202-z