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Molecular study and genotyping of Cryptosporidium baileyi and Cryptosporidium parvum from free-range and commercial broiler chickens in Guilan province, Iran
•C.baileyi and C.parvum are first molecularly detected in native free- range chickens from Iran.•C.parvum from poultries is genotyped for the first time in Iran.•A notable Cryptosporidium infection rate in the free-range and commercial broiler chickens in Iran was observed.•There was no significant...
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| Published in: | Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2020-04, Vol.69, p.101411-101411, Article 101411 |
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| container_title | Comparative immunology, microbiology and infectious diseases |
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| creator | Shahbazi, Parisa Aligolzadeh, Armin Khordadmehr, Monireh Hashemzadeh Farhang, Hosein Katiraee, Farzad |
| description | •C.baileyi and C.parvum are first molecularly detected in native free- range chickens from Iran.•C.parvum from poultries is genotyped for the first time in Iran.•A notable Cryptosporidium infection rate in the free-range and commercial broiler chickens in Iran was observed.•There was no significant difference between infection rate in native free-range and commercial broiler chickens.•The infection rate was significantly higher in chickens |
| doi_str_mv | 10.1016/j.cimid.2019.101411 |
| format | article |
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Cryptosporidiosis acutely impacts the digestive and/or respiratory tract of the birds in many species of various orders. More importantly, it is also well known as a significant zoonotic disease, which can lead to diarrhea in humans and livestock. Regarding increasing demand for free-range products and increasing the number of free-range poultry farms, the present paper evaluated histopathological and molecular detection of Cryptosporidium baileyi and Cryptosporidium parvum in free-range and commercial broiler chickens in the north part of Iran. For this purpose, 100 fecal and tissue samples of the chickens in Guilan province were collected. After microscopic examination using Ziehl-Neelsen staining, molecular analyses of the fecal samples were processed by Nested-PCR targeting the 18S rRNA gene followed by sequencing of the amplicons and phylogenetic analyses. Eventually, the tissue samples were studied for histological lesions. Findings demonstrated the presence of Cryptosporidium baileyi and Cryptosporidium parvum in 6 % and 2 % of fecal samples, respectively. This is the first identification of C.parvum in avian hosts in Iran, and for the first time, C.baileyi and C.parvum are shown in native free-range chickens in Iran. All of the PCR positive birds with clinical symptoms showed gross lesions of respiratory infections. There was no significant difference between infection rate in free-range and commercial broiler chickens; however, the infection rate was significantly higher in chickens <25 days old. To conclude, we present here a notable Cryptosporidium infection rate in the free-range chicks in Iran, which notify the role of this host as a reservoir and should be more noted due to the economic and zoonotic importance.</description><identifier>ISSN: 0147-9571</identifier><identifier>EISSN: 1878-1667</identifier><identifier>DOI: 10.1016/j.cimid.2019.101411</identifier><identifier>PMID: 31951878</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animal tissues ; Birds ; Broiler chickens ; C. baileyi ; C.parvum ; Chickens ; Chicks ; Cryptosporidiosis ; Cryptosporidium ; Cryptosporidium baileyi ; Cryptosporidium parvum ; Diarrhea ; Farms ; Feces ; Free-range ; Gene sequencing ; Genotyping ; Infections ; Lesions ; Livestock ; Phylogeny ; Poultry ; Poultry farming ; Protozoa ; Respiratory tract ; rRNA 18S ; Signs and symptoms ; Tissue analysis ; Zoonoses</subject><ispartof>Comparative immunology, microbiology and infectious diseases, 2020-04, Vol.69, p.101411-101411, Article 101411</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Apr 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-8520643256cb568b16642a61944c166598bd78f8a0a17e8f538de32636019ef13</citedby><cites>FETCH-LOGICAL-c387t-8520643256cb568b16642a61944c166598bd78f8a0a17e8f538de32636019ef13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31951878$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shahbazi, Parisa</creatorcontrib><creatorcontrib>Aligolzadeh, Armin</creatorcontrib><creatorcontrib>Khordadmehr, Monireh</creatorcontrib><creatorcontrib>Hashemzadeh Farhang, Hosein</creatorcontrib><creatorcontrib>Katiraee, Farzad</creatorcontrib><title>Molecular study and genotyping of Cryptosporidium baileyi and Cryptosporidium parvum from free-range and commercial broiler chickens in Guilan province, Iran</title><title>Comparative immunology, microbiology and infectious diseases</title><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><description>•C.baileyi and C.parvum are first molecularly detected in native free- range chickens from Iran.•C.parvum from poultries is genotyped for the first time in Iran.•A notable Cryptosporidium infection rate in the free-range and commercial broiler chickens in Iran was observed.•There was no significant difference between infection rate in native free-range and commercial broiler chickens.•The infection rate was significantly higher in chickens <25 days old
Cryptosporidiosis acutely impacts the digestive and/or respiratory tract of the birds in many species of various orders. More importantly, it is also well known as a significant zoonotic disease, which can lead to diarrhea in humans and livestock. Regarding increasing demand for free-range products and increasing the number of free-range poultry farms, the present paper evaluated histopathological and molecular detection of Cryptosporidium baileyi and Cryptosporidium parvum in free-range and commercial broiler chickens in the north part of Iran. For this purpose, 100 fecal and tissue samples of the chickens in Guilan province were collected. After microscopic examination using Ziehl-Neelsen staining, molecular analyses of the fecal samples were processed by Nested-PCR targeting the 18S rRNA gene followed by sequencing of the amplicons and phylogenetic analyses. Eventually, the tissue samples were studied for histological lesions. Findings demonstrated the presence of Cryptosporidium baileyi and Cryptosporidium parvum in 6 % and 2 % of fecal samples, respectively. This is the first identification of C.parvum in avian hosts in Iran, and for the first time, C.baileyi and C.parvum are shown in native free-range chickens in Iran. All of the PCR positive birds with clinical symptoms showed gross lesions of respiratory infections. There was no significant difference between infection rate in free-range and commercial broiler chickens; however, the infection rate was significantly higher in chickens <25 days old. To conclude, we present here a notable Cryptosporidium infection rate in the free-range chicks in Iran, which notify the role of this host as a reservoir and should be more noted due to the economic and zoonotic importance.</description><subject>Animal tissues</subject><subject>Birds</subject><subject>Broiler chickens</subject><subject>C. baileyi</subject><subject>C.parvum</subject><subject>Chickens</subject><subject>Chicks</subject><subject>Cryptosporidiosis</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium baileyi</subject><subject>Cryptosporidium parvum</subject><subject>Diarrhea</subject><subject>Farms</subject><subject>Feces</subject><subject>Free-range</subject><subject>Gene sequencing</subject><subject>Genotyping</subject><subject>Infections</subject><subject>Lesions</subject><subject>Livestock</subject><subject>Phylogeny</subject><subject>Poultry</subject><subject>Poultry farming</subject><subject>Protozoa</subject><subject>Respiratory tract</subject><subject>rRNA 18S</subject><subject>Signs and symptoms</subject><subject>Tissue analysis</subject><subject>Zoonoses</subject><issn>0147-9571</issn><issn>1878-1667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kcFu1DAQhi0EokvhCZCQJS4cyNaOY8c5cEArKJWKeoGz5diTxUtiBztZKQ_Du9bZLRx66MVjjb_5Zzw_Qm8p2VJCxdVha9zg7LYktFkzFaXP0IbKWhZUiPo52uRcXTS8phfoVUoHQkiTqZfogtGGr-AG_f0eejBzryNO02wXrL3Fe_BhWkbn9zh0eBeXcQppDNFZNw-41a6HxZ3Ix2-jjsccuhjWA6CI2u_hhJowDBCN0z1uY8gSEZtfzvwGn7Dz-Hp2vfZ4jOHovIGP-CaXvkYvOt0nePMQL9HPr19-7L4Vt3fXN7vPt4Vhsp4KyUsiKlZyYVouZJu_X5Va0KaqTL7zRra2lp3URNMaZMeZtMBKwUReHXSUXaIPZ93c_s8MaVKDSwb6PBGEOamSVVRQzoXI6PtH6CHM0efpMlXLpikrzjLFzpSJIaUInRqjG3RcFCVqdU8d1Mk9tbqnzu7lqncP2nM7gP1f88-uDHw6A5CXcXQQVTIO8rqsi2AmZYN7ssE98patgg</recordid><startdate>202004</startdate><enddate>202004</enddate><creator>Shahbazi, Parisa</creator><creator>Aligolzadeh, Armin</creator><creator>Khordadmehr, Monireh</creator><creator>Hashemzadeh Farhang, Hosein</creator><creator>Katiraee, Farzad</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>202004</creationdate><title>Molecular study and genotyping of Cryptosporidium baileyi and Cryptosporidium parvum from free-range and commercial broiler chickens in Guilan province, Iran</title><author>Shahbazi, Parisa ; Aligolzadeh, Armin ; Khordadmehr, Monireh ; Hashemzadeh Farhang, Hosein ; Katiraee, Farzad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-8520643256cb568b16642a61944c166598bd78f8a0a17e8f538de32636019ef13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animal tissues</topic><topic>Birds</topic><topic>Broiler chickens</topic><topic>C. baileyi</topic><topic>C.parvum</topic><topic>Chickens</topic><topic>Chicks</topic><topic>Cryptosporidiosis</topic><topic>Cryptosporidium</topic><topic>Cryptosporidium baileyi</topic><topic>Cryptosporidium parvum</topic><topic>Diarrhea</topic><topic>Farms</topic><topic>Feces</topic><topic>Free-range</topic><topic>Gene sequencing</topic><topic>Genotyping</topic><topic>Infections</topic><topic>Lesions</topic><topic>Livestock</topic><topic>Phylogeny</topic><topic>Poultry</topic><topic>Poultry farming</topic><topic>Protozoa</topic><topic>Respiratory tract</topic><topic>rRNA 18S</topic><topic>Signs and symptoms</topic><topic>Tissue analysis</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shahbazi, Parisa</creatorcontrib><creatorcontrib>Aligolzadeh, Armin</creatorcontrib><creatorcontrib>Khordadmehr, Monireh</creatorcontrib><creatorcontrib>Hashemzadeh Farhang, Hosein</creatorcontrib><creatorcontrib>Katiraee, Farzad</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shahbazi, Parisa</au><au>Aligolzadeh, Armin</au><au>Khordadmehr, Monireh</au><au>Hashemzadeh Farhang, Hosein</au><au>Katiraee, Farzad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular study and genotyping of Cryptosporidium baileyi and Cryptosporidium parvum from free-range and commercial broiler chickens in Guilan province, Iran</atitle><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><date>2020-04</date><risdate>2020</risdate><volume>69</volume><spage>101411</spage><epage>101411</epage><pages>101411-101411</pages><artnum>101411</artnum><issn>0147-9571</issn><eissn>1878-1667</eissn><abstract>•C.baileyi and C.parvum are first molecularly detected in native free- range chickens from Iran.•C.parvum from poultries is genotyped for the first time in Iran.•A notable Cryptosporidium infection rate in the free-range and commercial broiler chickens in Iran was observed.•There was no significant difference between infection rate in native free-range and commercial broiler chickens.•The infection rate was significantly higher in chickens <25 days old
Cryptosporidiosis acutely impacts the digestive and/or respiratory tract of the birds in many species of various orders. More importantly, it is also well known as a significant zoonotic disease, which can lead to diarrhea in humans and livestock. Regarding increasing demand for free-range products and increasing the number of free-range poultry farms, the present paper evaluated histopathological and molecular detection of Cryptosporidium baileyi and Cryptosporidium parvum in free-range and commercial broiler chickens in the north part of Iran. For this purpose, 100 fecal and tissue samples of the chickens in Guilan province were collected. After microscopic examination using Ziehl-Neelsen staining, molecular analyses of the fecal samples were processed by Nested-PCR targeting the 18S rRNA gene followed by sequencing of the amplicons and phylogenetic analyses. Eventually, the tissue samples were studied for histological lesions. Findings demonstrated the presence of Cryptosporidium baileyi and Cryptosporidium parvum in 6 % and 2 % of fecal samples, respectively. This is the first identification of C.parvum in avian hosts in Iran, and for the first time, C.baileyi and C.parvum are shown in native free-range chickens in Iran. All of the PCR positive birds with clinical symptoms showed gross lesions of respiratory infections. There was no significant difference between infection rate in free-range and commercial broiler chickens; however, the infection rate was significantly higher in chickens <25 days old. To conclude, we present here a notable Cryptosporidium infection rate in the free-range chicks in Iran, which notify the role of this host as a reservoir and should be more noted due to the economic and zoonotic importance.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>31951878</pmid><doi>10.1016/j.cimid.2019.101411</doi><tpages>1</tpages></addata></record> |
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| ispartof | Comparative immunology, microbiology and infectious diseases, 2020-04, Vol.69, p.101411-101411, Article 101411 |
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| source | ScienceDirect Freedom Collection |
| subjects | Animal tissues Birds Broiler chickens C. baileyi C.parvum Chickens Chicks Cryptosporidiosis Cryptosporidium Cryptosporidium baileyi Cryptosporidium parvum Diarrhea Farms Feces Free-range Gene sequencing Genotyping Infections Lesions Livestock Phylogeny Poultry Poultry farming Protozoa Respiratory tract rRNA 18S Signs and symptoms Tissue analysis Zoonoses |
| title | Molecular study and genotyping of Cryptosporidium baileyi and Cryptosporidium parvum from free-range and commercial broiler chickens in Guilan province, Iran |
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