Scaffold‐ and serum‐free hypertrophic cartilage tissue engineering as an alternative approach for bone repair

Human adipose stem/stromal cell (ASC) spheroids were used as a serum‐free in vitro model to recapitulate the molecular events and extracellular matrix organization that orchestrate a hypertrophic cartilage phenotype. Induced‐ASC spheroids (ø = 450 µm) showed high cell viability throughout the period...

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Bibliographic Details
Published in:Artificial organs 2020-07, Vol.44 (7), p.E288-E299
Main Authors: Kronemberger, Gabriela S., Dalmônico, Gisele M. L., Rossi, André L., Leite, Paulo Emílio Correa, Saraiva, Antonio M., Beatrici, Anderson, Silva, Karina Ribeiro, Granjeiro, José Mauro, Baptista, Leandra Santos
Format: Article
Language:English
Subjects:
adipose stem/stromal cells
Adipose Tissue - cytology
Alizarin
Alkaline phosphatase
Biomedical materials
bone
Bone healing
Calcium
Cartilage
Cartilage - cytology
Cartilage - growth & development
Cartilage - ultrastructure
Cell culture
Cell Differentiation - physiology
Cell viability
Cells, Cultured
Chondrogenesis - physiology
Collagen
Collagen (type I)
Collagen Type X - metabolism
Compression
Culture Media, Serum-Free
Endochondral bone
Eutrophication
Extracellular matrix
Extracellular Matrix - metabolism
Fibroblast growth factor 2
Gene expression
Humans
hypertrophic cartilage
Hypertrophy
Matrix Metalloproteinase 13 - metabolism
Matrix metalloproteinases
Mesenchymal Stem Cells - physiology
Metalloproteinase
Microscopy, Electron, Transmission
Ossification
Osteocalcin
Phenotypes
Primary Cell Culture - methods
RANTES
scaffold‐free
serum‐free
Spheroids
Spheroids, Cellular - physiology
Spheroids, Cellular - ultrastructure
Staining
Stromal Cells - physiology
Tenascin
Tenascin C
Tissue engineering
Tissue Engineering - methods
Vascular endothelial growth factor
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Summary:Human adipose stem/stromal cell (ASC) spheroids were used as a serum‐free in vitro model to recapitulate the molecular events and extracellular matrix organization that orchestrate a hypertrophic cartilage phenotype. Induced‐ASC spheroids (ø = 450 µm) showed high cell viability throughout the period of culture. The expression of collagen type X alpha 1 chain (COLXA1) and matrix metallopeptidase 13 (MMP‐13) was upregulated at week 2 in induced‐ASC spheroids compared with week 5 (P 
ISSN:0160-564X
1525-1594
DOI:10.1111/aor.13637