Genome-wide analysis of alternative splicing differences between oocyte and zygote

Alternative splicing (AS) of mRNA precursors allows the synthesis of multiple mRNAs from a single primary transcript, significantly expanding the information content and regulatory possibilities of higher eukaryotic genomes. During mammalian development, AS drives certain decisive changes in differe...

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Bibliographic Details
Published in:Biology of reproduction 2020-04, Vol.102 (5), p.999-1010
Main Authors: Cheng, Rui, Zheng, Xiaoman, Wang, Yingmei, Wang, Mengyun, Zhou, Chuan, Liu, Jun, Zhang, Yong, Quan, Fusheng, Liu, Xu
Format: Article
Language:English
Subjects:
alternative splicing
Cell cycle
Embryos
Gene expression
Genetic aspects
Genome-wide association studies
Genomes
Laboratory animals
mouse
Observations
oocyte
Oocytes
Physiological aspects
Polymerase chain reaction
Reproductive system
RESEARCH ARTICLE
RNA splicing
RNA-sequencing
Thermal cycling
Veterinary medicine
zygote
Zygotes
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Summary:Alternative splicing (AS) of mRNA precursors allows the synthesis of multiple mRNAs from a single primary transcript, significantly expanding the information content and regulatory possibilities of higher eukaryotic genomes. During mammalian development, AS drives certain decisive changes in different physiological processes. As development progresses, the maternal-to-zygotic transition (MZT) will trigger two processes: elimination of a subset of maternal mRNA and transcription of the zygote genome begins. Recent high-throughput technological advancements have facilitated genome-wide AS, whereas its analysis in mouse oocyte transition to the zygote stage has not been reported. We present a high-resolution global analysis of AS transitions and discovered extensive AS transitions between mouse oocyte and zygote. The difference of AS patterns was further confirmed using reverse transcription-polymerase chain reaction analysis. Many genes with specific AS events in mouse oocytes are differentially expressed between oocyte and zygote, but only a few genes with specific AS events in zygote are differentially expressed between oocyte and zygote. We provide a landscape of AS events in mouse oocyte and zygote. Our results advance the understanding of AS transitions during mouse fertilization and its potential functions for MZT and further development. Summary Sentence AS events that are differentially expressed between oocyte and zygote are important in the regulation of embryo development.
ISSN:0006-3363
1529-7268
DOI:10.1093/biolre/ioaa004