Association of intronic polymorphisms (rs1549339, rs13402242) and mRNA expression variations in PSMD1 gene in arsenic-exposed workers

Ubiquitin-proteasome system (UPS) gene, PSMD1 , is an important gene for neutralization of damaged and misfolded protein(s). The current study was designed to study the genetic and expression variations of PSMD1 gene as a consequence of arsenic exposure and its potential implications in arsenic indu...

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Published in:Environmental science and pollution research international 2020-04, Vol.27 (10), p.11425-11437
Main Authors: Ahmad, Saqiba, Arif, Bushra, Akram, Zertashia, Ahmed, Malik Waqar, Khan, Asad Ullah, Hussain, Muhammad Zahid, Rahman, Faisal, Kayani, Mahmood Akhtar, Mahjabeen, Ishrat
Format: Article
Language:English
Subjects:
Aquatic Pollution
Arsenic
Atmospheric Protection/Air Quality Control/Air Pollution
Earth and Environmental Science
Ecotoxicology
Environment
Environmental Chemistry
Environmental Health
Environmental science
Exposure
Gene expression
Genetic diversity
Kilns
Mutants
Mutation
Neutralization
Occupational exposure
Proteasomes
Protein folding
Research Article
Ubiquitin
Waste Water Technology
Water Management
Water Pollution Control
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Summary:Ubiquitin-proteasome system (UPS) gene, PSMD1 , is an important gene for neutralization of damaged and misfolded protein(s). The current study was designed to study the genetic and expression variations of PSMD1 gene as a consequence of arsenic exposure and its potential implications in arsenic induced diseases. In the present study, 250 blood samples of exposed industrial workers along with 250 controls were used. Initially, tetra amplification refractory mutation system-PCR was used to determine the role of PSMD1 gene polymorphisms (rs1549339, rs13402242) in industrial workers and controls. Frequency of homozygous mutant genotype of rs1549339 (OR: 2.23, 95% CI: 1.51–3.32, p  = 0.0001) and rs13402242 (OR: 2.96, 95% CI: 1.52–5.75, p  = 0.001) was observed significantly higher in exposed individuals vs controls. Secondly, qPCR was performed for expression analysis of PSMD1 gene. Significant down-regulated expression of PSMD1 gene ( p  
ISSN:0944-1344
1614-7499
DOI:10.1007/s11356-019-07422-x