Synthesis of a novel series of (Z)-3,5-disubstituted thiazolidine-2,4-diones as promising anti-breast cancer agents

• Published in: Bioorganic chemistry 2020-03, Vol.96, p.103569-103569, Article 103569
• Main Authors: El-Kashef, Hussein, Badr, Gamal, Abo El-Maali, Nagwa, Sayed, Douaa, Melnyk, Patricia, Lebegue, Nicolas, Abd El-Khalek, Rofida
• Format: Article
• Language: English
• Subjects: Anti-breast cancer; Antineoplastic Agents - chemical synthesis; Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; Apoptosis; Apoptosis - drug effects; Bcl-2 family; Breast Neoplasms - drug therapy; Breast Neoplasms - pathology; Cell Proliferation - drug effects; Female; Humans; MCF-7 Cells; Phosphorylation; Thiazolidine-2,4-diones; Thiazolidinediones; Thiazolidines - chemical synthesis; Thiazolidines - pharmacology; Thiazolidines - therapeutic use
• ISSN: 0045-2068; 1090-2120
• DOI: 10.1016/j.bioorg.2020.103569

[Display omitted] •Novel series of (Z)-3,5-disubstituted thiazolidine-2,4-diones has been synthesized.•Three of the synthesized compounds exert anti breast cancer effects.•They mediate growth arrest of breast cancer cell lines and human breast cancer cell.•These compounds alter the PI3K/AKT/mTOR, HIF, VEGF and Bcl-2 signaling pathways. A novel series of (Z)-3,5-disubstituted thiazolidine-2,4-diones 4–16 has been designed and synthesized. Preliminary screening of these compounds for their anti-breast cancer activity revealed that compounds 5, 7, and 9 possess the highest anti-cancer activities. The anti-tumor effects of compounds 5, 7, and 9 were evaluated against human breast cancer cell lines (MCF-7 and MDA-MB-231) and human breast cancer cells. They were also evaluated against normal non-cancerous breast cells, isolated from the same patients, to conclude about their use in a potential targeted therapy. Using MTT uptake method, these three compounds 5, 7, and 9 blunt the proliferation of these cancer cells in a dose-dependent manner with an IC50 of 1.27, 1.50 and 1.31 µM respectively. Interestingly, using flow cytometry analysis these three compounds significantly mediated apoptosis of human breast cancer cells without affecting the survival of normal non-cancerous breast cells that were isolated from the same patients. Mechanistically, these compounds blunt the proliferation of MCF-7 breast cancer cells by robustly decreasing the phosphorylation of AKT, mTOR and the expression of VEGF and HIF-1α. Most importantly, compounds 5, 7, and 9 without affecting the phosphorylation and expression of these crucial cellular factors in normal non-cancerous breast cells that were isolated from the same patients. Additionally, using Western blot analysis the three compounds significantly (P