Platform with biomimetic electrochemical sensors for adiponectin and leptin detection in human serum

• Published in: Talanta (Oxford) 2020-04, Vol.210, p.120643-120643, Article 120643
• Main Authors: Mihailescu, Carmen Marinela, Stan, Dana, Savin, Mihaela, Moldovan, Carmen Aura, Dinulescu, Silviu, Radulescu, Clara Hortensia, Firtat, Bogdan, Muscalu, George, Brasoveanu, Costin, Ion, Marian, Dragomir, David, Stan, Diana, Ion, Alina Catrinel
• Format: Article
• Language: English
• Subjects: Conductance; Impedance; Metabolic syndrome; Molecularly imprinted polymers; Polyphenol; Polyscopoletin
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2019.120643

Two new biomimetic sensors for the detection of adiponectin (A) and leptin (L) through molecularly imprinted polymers (MIPs) onto gold working electrodes (GWEs) were fabricated. Based on electrochemical impedance spectroscopy (EIS) results and cyclic voltammetry (CV) characteristics recorded in the development stages of the fabricated sensors, the sensors were electrochemically optimized and used in an integrated microfluidic platform to detect adiponectin/leptin via conductance signals and non-imprinted electrodes were used as references. To overcome the limitation of the low response signals after template binding non-conductive polyphenol (PP) and poliscopoletin (PS) were used for templates formation. Under optimized experimental conditions the conductance and resistance signals were obtained in the linear range of 0–50 μg ml−1 for A and 1–32 ng∙ml-1 for L with low limits of detection (0.25 μg ml−1 for A and 0.110 ng ml−1 for L). The dedicated platform exhibited an excellent response with great selectivity and stability. Finally, the proposed biomimetic sensors were successfully applied to enable the determination of A and L in human patient's serum with very high accuracy when compared to enzyme-linked immunosorbent assay ELISA reference methods. [Display omitted] •First report on biomimetic sensors for A and L detect from human serum.•The dedicated platform exhibited an excellent response with great selectivity.•Comparative electrochemical study regarding MIPA and MIPL.•An integrated microfluidic platform for detection of A and L was developed.•A and L patients values obtained with platform were compared with ELISA reference method.