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Tachykinin-1 receptor antagonism suppresses substance-P- and compound 48/80-induced mast cell activation from rat mast cells expressing functional mas-related GPCR B3
Objective Mice and rats are important animal models for mast cell (MC) study. However, rat Mas-related-GPCR-B3 receptor (MRGPRB3) has been less studied than its mouse counterpart. Therefore, we aimed to characterize rat MRGPRB3. Methods Mrgprb3 mRNA expression was assessed in peritoneal cells (RPCs)...
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| Published in: | Inflammation research 2020-03, Vol.69 (3), p.289-298 |
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| container_end_page | 298 |
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| container_title | Inflammation research |
| container_volume | 69 |
| creator | Sahid, Muhammad N. A. Liu, Shuang Mogi, Masaki Maeyama, Kazutaka |
| description | Objective
Mice and rats are important animal models for mast cell (MC) study. However, rat Mas-related-GPCR-B3 receptor (MRGPRB3) has been less studied than its mouse counterpart. Therefore, we aimed to characterize rat MRGPRB3.
Methods
Mrgprb3
mRNA expression was assessed in peritoneal cells (RPCs) and peritoneal MCs (RPMCs) of wild-type rats, RPCs of MC-deficient rats, and RBL-2H3 cells by reverse-transcriptase polymerase chain reaction (RT-PCR). RPMCs,
MRGPRX2
-transfected and non-transfected RBL-2H3 cells were activated by 15–30 min incubation with DNP-BSA, substance-P (SP), or compound-48/80. L732138 or CP96344 was used as a tachykinin/neurokinin-1-receptor antagonist. Histamine release from MCs was measured by HPLC fluorometry.
Results
Mrgprb3
mRNA expression was found in all cells, with the highest level in wild-type RPCs. All cells responded to DNP-BSA, but only
MRGPRX2
-transfected-RBL-2H3 cells and RPMCs responded to all activators. L732138 (0.1–10 μM) and CP96344 (1–100 μM) suppressed SP (10 μM)-induced RPMC activation. L732138 inhibition was dose independent, whereas CP96344 inhibition occurred in a dose-dependent manner. Additionally, only CP96344 suppressed SP (100 μM)- and compound-48/80 (10 μg/mL)-induced RPMC activation.
Conclusions
RPMCs expressing functional MRGPRB3 response upon MRGPRX2 ligands to regulated MC-mediated activities. It`s provide novel insights for future pseudo-allergic studies in rodents. |
| doi_str_mv | 10.1007/s00011-020-01319-z |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_2348230621</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2348230621</sourcerecordid><originalsourceid>FETCH-LOGICAL-p213t-85cf42c61ed8a8dd9b1bfdd41441e61dedc6cd2eb14d76f18be384d9f882bac33</originalsourceid><addsrcrecordid>eNpd0c9qFTEUBvAgFttefQEXEnDjJjb_7tzMUi9ahUJLacFdyCRnrqkzyZhkiu0D9TnN9FYKrnJCfnw58CH0ltGPjNLNSaaUMkYop4QywVpy_wIdMVmvLVU_XtaZckGEEvQQHed8U7niir9ChxW3otmsj9DDlbE_73754ANhOIGFqcSETShmF4PPI87zNCXIGXIdu1xMsEAuSCUO2zhOca6DVCeKEh_cbMHh0eSCLQwDNrb4W1N8DLhPccTJlOfXjOHPY7QPO9zPwS7ODAsgCQZTatTpxfYSfxav0UFvhgxvns4Vuv765Wr7jZydn37ffjojE2eiELW2veS2YeCUUc61Het65ySTkkHDHDjbWMehY9Jtmp6pDoSSru2V4p2xQqzQh33ulOLvGXLRo8_LriZAnLPmQiouaFN_W6H3_9GbOKe6_6LW6-oa0VT17knN3QhOT8mPJt3pfw1UIPYg16ewg_Qcw6heetb7nnXtWT_2rO_FX5_Rm3o</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2355482636</pqid></control><display><type>article</type><title>Tachykinin-1 receptor antagonism suppresses substance-P- and compound 48/80-induced mast cell activation from rat mast cells expressing functional mas-related GPCR B3</title><source>Springer Link</source><creator>Sahid, Muhammad N. A. ; Liu, Shuang ; Mogi, Masaki ; Maeyama, Kazutaka</creator><creatorcontrib>Sahid, Muhammad N. A. ; Liu, Shuang ; Mogi, Masaki ; Maeyama, Kazutaka</creatorcontrib><description>Objective
Mice and rats are important animal models for mast cell (MC) study. However, rat Mas-related-GPCR-B3 receptor (MRGPRB3) has been less studied than its mouse counterpart. Therefore, we aimed to characterize rat MRGPRB3.
Methods
Mrgprb3
mRNA expression was assessed in peritoneal cells (RPCs) and peritoneal MCs (RPMCs) of wild-type rats, RPCs of MC-deficient rats, and RBL-2H3 cells by reverse-transcriptase polymerase chain reaction (RT-PCR). RPMCs,
MRGPRX2
-transfected and non-transfected RBL-2H3 cells were activated by 15–30 min incubation with DNP-BSA, substance-P (SP), or compound-48/80. L732138 or CP96344 was used as a tachykinin/neurokinin-1-receptor antagonist. Histamine release from MCs was measured by HPLC fluorometry.
Results
Mrgprb3
mRNA expression was found in all cells, with the highest level in wild-type RPCs. All cells responded to DNP-BSA, but only
MRGPRX2
-transfected-RBL-2H3 cells and RPMCs responded to all activators. L732138 (0.1–10 μM) and CP96344 (1–100 μM) suppressed SP (10 μM)-induced RPMC activation. L732138 inhibition was dose independent, whereas CP96344 inhibition occurred in a dose-dependent manner. Additionally, only CP96344 suppressed SP (100 μM)- and compound-48/80 (10 μg/mL)-induced RPMC activation.
Conclusions
RPMCs expressing functional MRGPRB3 response upon MRGPRX2 ligands to regulated MC-mediated activities. It`s provide novel insights for future pseudo-allergic studies in rodents.</description><identifier>ISSN: 1023-3830</identifier><identifier>EISSN: 1420-908X</identifier><identifier>DOI: 10.1007/s00011-020-01319-z</identifier><identifier>PMID: 31993675</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Allergology ; Amino acids ; Animal models ; Animals ; Antibiotics ; Biomedical and Life Sciences ; Biomedicine ; Cell activation ; Cell culture ; Cell Degranulation - drug effects ; Compound 48/80 ; Dermatology ; Dinitrophenols ; Fluorimetry ; Fluorometry ; G protein-coupled receptors ; Gene expression ; Genes ; High-performance liquid chromatography ; Histamine ; Histamine Release - drug effects ; Humans ; Immunology ; Inflammation ; Inflammation - drug therapy ; Ligands ; Liquid chromatography ; Male ; Mast cells ; Mast Cells - drug effects ; Mast Cells - immunology ; Mice ; Nerve Tissue Proteins - metabolism ; Neurokinin ; Neurokinin NK1 receptors ; Neurokinin-1 Receptor Antagonists - pharmacology ; Neurology ; Original Research Paper ; p-Methoxy-N-methylphenethylamine - pharmacology ; Peptides ; Peritoneum ; Pharmacology/Toxicology ; Polymerase chain reaction ; Protein Conformation ; Rats ; Receptors, G-Protein-Coupled - metabolism ; Receptors, Neuropeptide - metabolism ; Rheumatology ; Serum Albumin, Bovine ; Substance P - pharmacology ; Tachykinin ; Tachykinin receptors ; Tachykinins - chemistry</subject><ispartof>Inflammation research, 2020-03, Vol.69 (3), p.289-298</ispartof><rights>Springer Nature Switzerland AG 2020</rights><rights>Inflammation Research is a copyright of Springer, (2020). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-p213t-85cf42c61ed8a8dd9b1bfdd41441e61dedc6cd2eb14d76f18be384d9f882bac33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31993675$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sahid, Muhammad N. A.</creatorcontrib><creatorcontrib>Liu, Shuang</creatorcontrib><creatorcontrib>Mogi, Masaki</creatorcontrib><creatorcontrib>Maeyama, Kazutaka</creatorcontrib><title>Tachykinin-1 receptor antagonism suppresses substance-P- and compound 48/80-induced mast cell activation from rat mast cells expressing functional mas-related GPCR B3</title><title>Inflammation research</title><addtitle>Inflamm. Res</addtitle><addtitle>Inflamm Res</addtitle><description>Objective
Mice and rats are important animal models for mast cell (MC) study. However, rat Mas-related-GPCR-B3 receptor (MRGPRB3) has been less studied than its mouse counterpart. Therefore, we aimed to characterize rat MRGPRB3.
Methods
Mrgprb3
mRNA expression was assessed in peritoneal cells (RPCs) and peritoneal MCs (RPMCs) of wild-type rats, RPCs of MC-deficient rats, and RBL-2H3 cells by reverse-transcriptase polymerase chain reaction (RT-PCR). RPMCs,
MRGPRX2
-transfected and non-transfected RBL-2H3 cells were activated by 15–30 min incubation with DNP-BSA, substance-P (SP), or compound-48/80. L732138 or CP96344 was used as a tachykinin/neurokinin-1-receptor antagonist. Histamine release from MCs was measured by HPLC fluorometry.
Results
Mrgprb3
mRNA expression was found in all cells, with the highest level in wild-type RPCs. All cells responded to DNP-BSA, but only
MRGPRX2
-transfected-RBL-2H3 cells and RPMCs responded to all activators. L732138 (0.1–10 μM) and CP96344 (1–100 μM) suppressed SP (10 μM)-induced RPMC activation. L732138 inhibition was dose independent, whereas CP96344 inhibition occurred in a dose-dependent manner. Additionally, only CP96344 suppressed SP (100 μM)- and compound-48/80 (10 μg/mL)-induced RPMC activation.
Conclusions
RPMCs expressing functional MRGPRB3 response upon MRGPRX2 ligands to regulated MC-mediated activities. It`s provide novel insights for future pseudo-allergic studies in rodents.</description><subject>Allergology</subject><subject>Amino acids</subject><subject>Animal models</subject><subject>Animals</subject><subject>Antibiotics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell activation</subject><subject>Cell culture</subject><subject>Cell Degranulation - drug effects</subject><subject>Compound 48/80</subject><subject>Dermatology</subject><subject>Dinitrophenols</subject><subject>Fluorimetry</subject><subject>Fluorometry</subject><subject>G protein-coupled receptors</subject><subject>Gene expression</subject><subject>Genes</subject><subject>High-performance liquid chromatography</subject><subject>Histamine</subject><subject>Histamine Release - drug effects</subject><subject>Humans</subject><subject>Immunology</subject><subject>Inflammation</subject><subject>Inflammation - drug therapy</subject><subject>Ligands</subject><subject>Liquid chromatography</subject><subject>Male</subject><subject>Mast cells</subject><subject>Mast Cells - drug effects</subject><subject>Mast Cells - immunology</subject><subject>Mice</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neurokinin</subject><subject>Neurokinin NK1 receptors</subject><subject>Neurokinin-1 Receptor Antagonists - pharmacology</subject><subject>Neurology</subject><subject>Original Research Paper</subject><subject>p-Methoxy-N-methylphenethylamine - pharmacology</subject><subject>Peptides</subject><subject>Peritoneum</subject><subject>Pharmacology/Toxicology</subject><subject>Polymerase chain reaction</subject><subject>Protein Conformation</subject><subject>Rats</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>Receptors, Neuropeptide - metabolism</subject><subject>Rheumatology</subject><subject>Serum Albumin, Bovine</subject><subject>Substance P - pharmacology</subject><subject>Tachykinin</subject><subject>Tachykinin receptors</subject><subject>Tachykinins - chemistry</subject><issn>1023-3830</issn><issn>1420-908X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpd0c9qFTEUBvAgFttefQEXEnDjJjb_7tzMUi9ahUJLacFdyCRnrqkzyZhkiu0D9TnN9FYKrnJCfnw58CH0ltGPjNLNSaaUMkYop4QywVpy_wIdMVmvLVU_XtaZckGEEvQQHed8U7niir9ChxW3otmsj9DDlbE_73754ANhOIGFqcSETShmF4PPI87zNCXIGXIdu1xMsEAuSCUO2zhOca6DVCeKEh_cbMHh0eSCLQwDNrb4W1N8DLhPccTJlOfXjOHPY7QPO9zPwS7ODAsgCQZTatTpxfYSfxav0UFvhgxvns4Vuv765Wr7jZydn37ffjojE2eiELW2veS2YeCUUc61Het65ySTkkHDHDjbWMehY9Jtmp6pDoSSru2V4p2xQqzQh33ulOLvGXLRo8_LriZAnLPmQiouaFN_W6H3_9GbOKe6_6LW6-oa0VT17knN3QhOT8mPJt3pfw1UIPYg16ewg_Qcw6heetb7nnXtWT_2rO_FX5_Rm3o</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Sahid, Muhammad N. A.</creator><creator>Liu, Shuang</creator><creator>Mogi, Masaki</creator><creator>Maeyama, Kazutaka</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20200301</creationdate><title>Tachykinin-1 receptor antagonism suppresses substance-P- and compound 48/80-induced mast cell activation from rat mast cells expressing functional mas-related GPCR B3</title><author>Sahid, Muhammad N. A. ; Liu, Shuang ; Mogi, Masaki ; Maeyama, Kazutaka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p213t-85cf42c61ed8a8dd9b1bfdd41441e61dedc6cd2eb14d76f18be384d9f882bac33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Allergology</topic><topic>Amino acids</topic><topic>Animal models</topic><topic>Animals</topic><topic>Antibiotics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell activation</topic><topic>Cell culture</topic><topic>Cell Degranulation - drug effects</topic><topic>Compound 48/80</topic><topic>Dermatology</topic><topic>Dinitrophenols</topic><topic>Fluorimetry</topic><topic>Fluorometry</topic><topic>G protein-coupled receptors</topic><topic>Gene expression</topic><topic>Genes</topic><topic>High-performance liquid chromatography</topic><topic>Histamine</topic><topic>Histamine Release - drug effects</topic><topic>Humans</topic><topic>Immunology</topic><topic>Inflammation</topic><topic>Inflammation - drug therapy</topic><topic>Ligands</topic><topic>Liquid chromatography</topic><topic>Male</topic><topic>Mast cells</topic><topic>Mast Cells - drug effects</topic><topic>Mast Cells - immunology</topic><topic>Mice</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neurokinin</topic><topic>Neurokinin NK1 receptors</topic><topic>Neurokinin-1 Receptor Antagonists - pharmacology</topic><topic>Neurology</topic><topic>Original Research Paper</topic><topic>p-Methoxy-N-methylphenethylamine - pharmacology</topic><topic>Peptides</topic><topic>Peritoneum</topic><topic>Pharmacology/Toxicology</topic><topic>Polymerase chain reaction</topic><topic>Protein Conformation</topic><topic>Rats</topic><topic>Receptors, G-Protein-Coupled - metabolism</topic><topic>Receptors, Neuropeptide - metabolism</topic><topic>Rheumatology</topic><topic>Serum Albumin, Bovine</topic><topic>Substance P - pharmacology</topic><topic>Tachykinin</topic><topic>Tachykinin receptors</topic><topic>Tachykinins - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sahid, Muhammad N. A.</creatorcontrib><creatorcontrib>Liu, Shuang</creatorcontrib><creatorcontrib>Mogi, Masaki</creatorcontrib><creatorcontrib>Maeyama, Kazutaka</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Inflammation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sahid, Muhammad N. A.</au><au>Liu, Shuang</au><au>Mogi, Masaki</au><au>Maeyama, Kazutaka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tachykinin-1 receptor antagonism suppresses substance-P- and compound 48/80-induced mast cell activation from rat mast cells expressing functional mas-related GPCR B3</atitle><jtitle>Inflammation research</jtitle><stitle>Inflamm. Res</stitle><addtitle>Inflamm Res</addtitle><date>2020-03-01</date><risdate>2020</risdate><volume>69</volume><issue>3</issue><spage>289</spage><epage>298</epage><pages>289-298</pages><issn>1023-3830</issn><eissn>1420-908X</eissn><abstract>Objective
Mice and rats are important animal models for mast cell (MC) study. However, rat Mas-related-GPCR-B3 receptor (MRGPRB3) has been less studied than its mouse counterpart. Therefore, we aimed to characterize rat MRGPRB3.
Methods
Mrgprb3
mRNA expression was assessed in peritoneal cells (RPCs) and peritoneal MCs (RPMCs) of wild-type rats, RPCs of MC-deficient rats, and RBL-2H3 cells by reverse-transcriptase polymerase chain reaction (RT-PCR). RPMCs,
MRGPRX2
-transfected and non-transfected RBL-2H3 cells were activated by 15–30 min incubation with DNP-BSA, substance-P (SP), or compound-48/80. L732138 or CP96344 was used as a tachykinin/neurokinin-1-receptor antagonist. Histamine release from MCs was measured by HPLC fluorometry.
Results
Mrgprb3
mRNA expression was found in all cells, with the highest level in wild-type RPCs. All cells responded to DNP-BSA, but only
MRGPRX2
-transfected-RBL-2H3 cells and RPMCs responded to all activators. L732138 (0.1–10 μM) and CP96344 (1–100 μM) suppressed SP (10 μM)-induced RPMC activation. L732138 inhibition was dose independent, whereas CP96344 inhibition occurred in a dose-dependent manner. Additionally, only CP96344 suppressed SP (100 μM)- and compound-48/80 (10 μg/mL)-induced RPMC activation.
Conclusions
RPMCs expressing functional MRGPRB3 response upon MRGPRX2 ligands to regulated MC-mediated activities. It`s provide novel insights for future pseudo-allergic studies in rodents.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>31993675</pmid><doi>10.1007/s00011-020-01319-z</doi><tpages>10</tpages></addata></record> |
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| subjects | Allergology Amino acids Animal models Animals Antibiotics Biomedical and Life Sciences Biomedicine Cell activation Cell culture Cell Degranulation - drug effects Compound 48/80 Dermatology Dinitrophenols Fluorimetry Fluorometry G protein-coupled receptors Gene expression Genes High-performance liquid chromatography Histamine Histamine Release - drug effects Humans Immunology Inflammation Inflammation - drug therapy Ligands Liquid chromatography Male Mast cells Mast Cells - drug effects Mast Cells - immunology Mice Nerve Tissue Proteins - metabolism Neurokinin Neurokinin NK1 receptors Neurokinin-1 Receptor Antagonists - pharmacology Neurology Original Research Paper p-Methoxy-N-methylphenethylamine - pharmacology Peptides Peritoneum Pharmacology/Toxicology Polymerase chain reaction Protein Conformation Rats Receptors, G-Protein-Coupled - metabolism Receptors, Neuropeptide - metabolism Rheumatology Serum Albumin, Bovine Substance P - pharmacology Tachykinin Tachykinin receptors Tachykinins - chemistry |
| title | Tachykinin-1 receptor antagonism suppresses substance-P- and compound 48/80-induced mast cell activation from rat mast cells expressing functional mas-related GPCR B3 |
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