The Contribution of Photodynamic Inactivation vs. Corsodyl Mouthwash to the Control of Streptococcus mutans Biofilms

This work compared the inhibition effect of the commercially available mouthwash Corsodyl, containing 0.1% chlorhexidine digluconate, and photodynamic inactivation (PDI) employing methylene blue (MB) with irradiation from a red laser on 24-h biofilms formed by Streptococcus mutans strains on hydroxy...

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Bibliographic Details
Published in:Current microbiology 2020-06, Vol.77 (6), p.988-996
Main Authors: Legéňová, Kornélia, Kovalčíková, Mária, Černáková, Lucia, Bujdáková, Helena
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Antimicrobial agents
Biofilms
Biofilms - drug effects
Biofilms - growth & development
Biomedical and Life Sciences
Biotechnology
Chlorhexidine
Chlorhexidine - analogs & derivatives
Chlorhexidine - analysis
Chlorhexidine - pharmacology
Colony Count, Microbial
Cytotoxicity
Deactivation
Dental caries
Durapatite
Hydroxyapatite
Inactivation
Irradiation
Larva - drug effects
Larvae
Laser microscopy
Lasers
Life Sciences
Methylene blue
Methylene Blue - pharmacology
Methylene Blue - radiation effects
Microbial Viability - drug effects
Microbiology
Moths - drug effects
Mouthwashes
Mouthwashes - chemistry
Mouthwashes - pharmacology
Photosensitizing Agents - pharmacology
Streptococcus infections
Streptococcus mutans
Streptococcus mutans - drug effects
Streptococcus mutans - physiology
Survival
Toxicity
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Summary:This work compared the inhibition effect of the commercially available mouthwash Corsodyl, containing 0.1% chlorhexidine digluconate, and photodynamic inactivation (PDI) employing methylene blue (MB) with irradiation from a red laser on 24-h biofilms formed by Streptococcus mutans strains on hydroxyapatite surfaces. The cytotoxicity of Corsodyl and MB was evaluated by Galleria mellonella surviving assay. The viability of biofilm cells after exposure to mouthwash and PDI was determined by counting colony-forming units. The inhibitory effect of antimicrobial agents was confirmed by confocal scanning laser microscopy. MB did not exhibit a cytotoxic effect on larval survival. Non-diluted Corsodyl slightly decreased the survival of larvae. Using our PDI parameters achieved better inhibition than with non-PDI, proving a significant effect on the eradication of S. mutans biofilms and therefore could be an appropriate supplement for the eradication of dental caries.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-020-01901-y